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目的探讨喉癌与HPV、EBV阳性表达的关系。方法采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)和实时荧光定量PCR技术对101例喉癌石蜡包埋组织标本进行37种HPV亚型检测和EBV定量检测,以43例声带息肉标本为对照。结果喉癌组HPV阳性率13.86%,对照组HPV阳性率9.3%,HPV在喉癌组和对照组的阳性表达差异无统计学意义(P>0.05);喉癌组EBV阳性率63.37%,对照组EBV阳性率13.95%,EBV在喉癌组和对照组的阳性表达差异具有统计学意义(P<0.05);通过不同分化程度喉癌组织中EBV阳性率的比较,EBV在高分化型与中分化型喉癌组织中阳性表达差异无统计学意义(P>0.05),EBV在高分化型与低分化型、中分化型与低分化型喉癌组织中阳性表达差异具有统计学意义(P<0.05);不同性别、年龄、病程的喉癌患者中EBV阳性率差异无统计学意义(P>0.05)。结论喉癌的发病与EBV感染密切相关,与HPV可能有关,HPV高危亚型感染在诱发喉癌的过程中有一定作用;喉癌患者的性别、年龄、病程长短与EBV感染无明显关系。本研究将为喉癌的病毒学致病机制及临床上防治喉癌提供一定的依据。
Objective To investigate the relationship between the positive expression of HPV and EBV in laryngeal cancer. Methods The genotypes of 37 HPV subtypes and EBV in 101 laryngeal squamous cell carcinomas were detected by Hybri Max and real-time fluorescence quantitative PCR (PCR-RFLP). 43 cases of vocal cord polyps Specimens for the control. Results The positive rate of HPV in laryngeal carcinoma was 13.86%, the positive rate of HPV in control group was 9.3%. The positive expression of HPV in laryngocarcinoma group and control group had no significant difference (P> 0.05). The positive rate of EBV in laryngeal carcinoma was 63.37% The positive rate of EBV was 13.95%. The positive expression rate of EBV in laryngeal carcinoma and control group was statistically significant (P <0.05). Compared with the positive rate of EBV in different degree of laryngeal carcinoma, The positive expression of EBV in differentiated laryngeal carcinoma was not statistically significant (P> 0.05). The positive expression of EBV in well-differentiated and poorly differentiated, poorly differentiated and poorly differentiated laryngeal carcinoma was statistically significant (P < 0.05). There was no significant difference in the positive rate of EBV between laryngeal cancer patients with different sex, age and course of disease (P> 0.05). Conclusions The incidence of laryngeal carcinoma is closely related to EBV infection and may be related to HPV. High risk HPV subtype infection plays a role in the carcinogenesis of laryngeal carcinoma. The gender, age and duration of laryngeal cancer have no significant relationship with EBV infection. This study will provide a basis for the pathogenesis of laryngeal virology and clinical prevention and treatment of laryngeal cancer.