论文部分内容阅读
目的:用气相色谱-质谱法对山东平邑及河南南阳产佩兰挥发油进行化学成分的分析。方法:采用水蒸气蒸馏法从2种佩兰中提取挥发油。采用不同类型的毛细管柱进行分析,找出最佳分析条件,用归一化法测定其百分含量,并用气相色谱-质谱法对化学成分进行鉴定。色谱条件:SE-54毛细管柱(25m×0.25mm,0.25μm),柱温90℃(7min)5℃·min~(-1) 260℃(10 min);分流进样,分流比1:50;进样温度对270℃,FID检测器,温度为270℃。结果:山东平邑产佩兰共鉴定了38个成分,占挥发油总成分的82％以上;河南南阳产佩兰共鉴定了39个成分,占挥发油总成分的82％以上。结论:本方法稳定可靠,重现性好,适用于中药挥发油的化学成分分析。
OBJECTIVE: To analyze the chemical constituents of Perennial oil produced in Pingyi, Shandong and Nanyang, Henan by gas chromatography-mass spectrometry. Methods: Volatile oils were extracted from two kinds of Perrin by steam distillation. Different types of capillary columns were used for analysis to find out the best analytical conditions. The percentages were determined by normalization and chemical components were identified by gas chromatography-mass spectrometry. Chromatographic conditions: SE-54 capillary column (25m × 0.25mm, 0.25μm), column temperature 90°C (7min) 5°C · min -1 260°C (10 min); split injection, split ratio 1:50 The injection temperature was 270°C and the FID detector was at 270°C. RESULTS: A total of 38 components were identified in Peilan, Shandong Province, accounting for more than 82% of the total volatile oil components; 39 components were identified in Peran, Henan, Nanyang, accounting for more than 82% of the total volatile oil components. Conclusion: The method is stable and reliable with good reproducibility. It is suitable for the analysis of chemical constituents of volatile oil from traditional Chinese medicine.