论文部分内容阅读
分离鉴定一株侵染烟草青枯雷尔氏菌的烈性噬菌体,观察其形态特征,并进行全基因组测序与分析,为采用噬菌体疗法防治烟草青枯病提供技术储备。以烟草青枯菌TB15-15为宿主菌,从土壤中筛选分离得到噬菌体;分离得到的噬菌体经纯化浓缩,采用透射电子显微镜观察其形态特征;提取噬菌体DNA,进行全基因组高通量测序,分析其全基因组的结构特征,比较基因组分析明确其进化关系。以TB15-15为宿主菌,从长期种植烟田土壤中分离得到一株青枯雷尔氏菌烈性噬菌体。电镜观察显示,该噬菌体属于有尾噬菌体目、短尾噬菌体科。基因组全长42 042bp,GC含量为63.2%,含有46个开放阅读框,比较基因组分析确定该噬菌体为一株新的青枯雷尔氏菌烈性噬菌体,命名为RS-PⅡ-1。以烟草青枯菌为宿主菌,分离到一株新的青枯雷尔氏菌烈性噬菌体RS-PⅡ-1,明确了其形态和基因组特征,为防治青枯病的噬菌体疗法奠定基础。
Isolation and identification of a strong bacteriophages infected with R. solanacearum, observation of its morphological characteristics, and genome-wide sequencing and analysis, for the use of bacteriophage therapy to provide technical reserves of tobacco bacterial wilt. Bacteriocin was screened and isolated from soil with R. solanacearum TB15-15 as host bacteria. The isolated phage were purified and concentrated, and their morphological characteristics were observed by transmission electron microscopy. The phage DNA was extracted and genome-wide high-throughput sequencing was carried out to analyze The whole genome of its structural characteristics, comparative genomics analysis of its evolutionary relationship. Taking TB15-15 as the host bacteria, a strong R. solanacearum phage was isolated from long-term tobacco soil. Electron microscopy showed that the phage belonged to the order of phage phage and the phage phage. The genome was 42 042 bp in length with a GC content of 63.2% and contained 46 open reading frames. Genomic analysis confirmed that the phage was a new strong R. solanacearum bacteriophage named RS-PⅡ-1. A new strain of Ralstonia solanacearum, potent bacteriophage RS-PⅡ-1, was isolated from R. solanacearum and its morphology and genomic characteristics were identified. This study laid the foundation for bacteriophage therapy against bacterial wilt.