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本研究通过选择适宜反应温度,合成具有适宜pH/温度敏感性的聚氨基胺-聚乙二醇-聚氨基胺三嵌段共聚物(PAA-PEG-PAA),制备PAA-PEG-PAA凝胶并对其性质进行考察;选择阿霉素为模型药物,制备含阿霉素的PAA-PEG-PAA凝胶并对其释放进行研究;选择B16F10黑色素瘤细胞为模型,考察含阿霉素的PAA-PEG-PAA凝胶的细胞药效.实验结果表明,通过降低反应温度,可降低PAA的分子量,使PAA-PEG-PAA具有适宜的pH/温度敏感性.PAA-PEG-PAA凝胶的粘度具有浓度依赖和温度依赖的特征.在pH 6.0到7.4的范围内,PAA-PEG-PAA凝胶(12％,w/w)的液体-凝固相转变温度为35到29 ℃.含阿霉素的PAA-PEG-PAA凝胶具有体外药物释放缓释的特征及体外抗肿瘤药效的作用.所制备的含阿霉素的PAA-PEG-PAA凝胶具有响应肿瘤酸性微环境及体温发生凝固、缓慢释放药物的特点,推测经肿瘤内或者肿瘤旁注射后,可发挥相应的抗肿瘤药效.“,”Many pH-and temperature-responsive polymers have been designed for preparing hydrogel.In the present study,in order to decrease the pH sensitivity of reported poly(amidoamine)-poly(ethyleneglycol)-poly(amidoamine) (PAA1580-PEGa600-PAA1580),we designed and synthesized poly(amidoamine)-poly(ethyleneglycol)-poly(amidoamine) (PAA-PEG-PAA) with shorter length of PAA chain by decreasing reaction temperature for preparing PAA-PEG-PAA hydrogel solution containing doxorubicin (DOX).The PAA-PEG-PAA was synthesized via the Michael-addition polymerization.The characteristic of PAA-PEG-PAA was evaluated.The PAA-PEG-PAA hydrogel solution was prepared and investigated.DOX-loaded PAA-PEG-PAA hydrogel solution was prepared,and its in vitro DOX release and in vitro anti-tumor activity were evaluated.Our results indicated that the viscosity of PAA-PEG-PAA hydrogel solution was concentration-and temperature-dependent.The sol-gel transition temperature of PAA-PEG-PAA hydrogel solution (12 ％,w/w) ranged from 35 to 29 ℃,and its pH ranged from 6.0 to 7.4.The released DOX from DOX-loaded PAA-PEG-PAA hydrogel showed sustained release characteristics.The in vitro anti-tumor activity of DOX-loaded PAA-PEG-PAA hydrogel was confirmed in B 16F 10 cell line.Considering the acidic tumor microenvironment,this DOX-loaded PAA-PEG-PAA hydrogel solution would be easy in situ administration for intra-tumor injection or para-tumor injection forming hydrogel at body temperature.We suggested that this DOX-loaded PAA-PEG-PAAhydrogel solution,if containing photothermal conversion agents,would have a potential further use for photothermal therapy.