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为探讨脾虚证时生长抑素分泌失常的机理，用Ｗｉｓｔａｒ雄性大鼠２４只，分为正常对照组、实验性脾虚组、自然恢复组和中药治疗组。４组动物同时处死，取胃制成恒冷箱切片，以地高辛标记的生长抑素ｃＲＮＡ为探针，用原位杂交法显示胃粘膜Ｄ细胞中的生长抑素ｍＲＮＡ，并对细胞内杂交信号进行了显微分光光度计测定。结果表明，与对照组相比，脾虚组胃粘膜Ｄ细胞ｍＲＮＡ表达增强，细胞密度增大。与自然恢复组相比，中药治疗组的Ｄ细胞ｍＲＮＡ的表达和细胞密度与对照组相近。本研究提示，脾虚证大鼠胃粘膜Ｄ细胞生长抑素ｍＲＮＡ的高表达导致生长抑素合成增高，并对其意义进行了讨论
In order to explore the mechanism of somatostatin secretion in spleen deficiency syndrome, 24 Wistar male rats were divided into normal control group, experimental spleen deficiency group, natural recovery group and traditional Chinese medicine treatment group. Four groups of animals were sacrificed at the same time and were made into constant cold box sections. The somatostatin cRNA labeled with digoxigenin was used as a probe to detect somatostatin mRNA in gastric mucosal D cells by in situ hybridization. Hybridization signals were measured by a spectrophotometer. The results showed that compared with the control group, the gastric mucosal D cells mRNA expression increased and the cell density increased in the spleen deficiency group. Compared with the natural recovery group, D-cell mRNA expression and cell density in the TCM-treated group were similar to those in the control group. The present study suggests that the high expression of somatostatin mRNA in gastric mucosal D cells of spleen deficiency rats leads to the increased synthesis of somatostatin, and its significance is discussed