角质形成细胞有一个较大的缺点 :用常规方法很难被外源基因转染 ,在正常角质形成细胞中建立稳定的转染更是非常困难。为了克服这些困难 ,作者运用了一种腺病毒载体ＡＸ。用ＡＸ腺病毒ＤＮＡ -终末蛋白复合体与粘粒盒共转染人胚胎肾 2 93细胞 ,该细胞产生目的重组病毒的效率是常 One of the major drawbacks of keratinocytes is that they are difficult to transfect with foreign genes by conventional methods and it is very difficult to establish stable transfection in normal keratinocytes. To overcome these difficulties, the authors used an adenovirus vector, AX. Human embryonic kidney 293 cells are co-transfected with the AX adenovirus DNA-terminal protein complex and the cosmid cassette and the efficiency of this cell to produce the desired recombinant virus is constant