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[目的]探讨在兔二次打击多器官功能障碍综合征(MODS)动物模型中PPAR-γ、NF-κB、TNF-α、IL-10的表达,血气及生化指标的变化以及罗格列酮及GW9662对其的影响.[方法]在失血性损伤的基础上以脂多糖(LPS)腹腔注射作为二次打击制备兔MODS模型,使用PPAR-γ的激动剂罗格列酮和拮抗剂GW9662进行干预,测定24 h后PPAR-γ、NF-κB、TNF-α、IL-10在肝脏中的表达以及血气及生化指标的变化.[结果]T-H组与D-H-S组较之sham组中PPAR-γmRNA的表达下降,具有显著差异性(P<0.05).在D-H组中,罗格列酮亚组较空白组明显升高,具有显著差异性(P<0.05).GW9662亚组较之罗格列酮亚组明显下降,具有显著差异性(P<0.05).在T-H组较之于sham组,NF-κB、TNF-α的含量增加且具有显著性(P0.05).在D-H-S组中NF-κB、TNF-α的含量较T-H组明显增加(P<0.05),IL-10较sham组明显升高(P0.05).在D-H-R组,NF-κB、TNF-α的含量较D-H-S组明显减少(P<0.05),IL-10的含量较D-H-S组明显增加(P<0.05).在D-H-G组NF-κB、TNF-α的含量较D-H-R组明显增加(P<0.05),IL-10的含量较D-H-R组明显减少(P<0.05).在未使用PPAR-γ激动剂或拮抗剂的情况下,T-H组中血气及生化指标较之sham组均没有明显改变,在D-H组中血气及生化指标较之sham组及T-H组均明显升高(P<0.05).在D-H组中罗格列酮组较空白组血气及生化指标明显下降(P<0.05),GW9662组较罗格列酮组血气及生化指标明显升高(P<0.05).[结论]PPAR-γ的激动剂罗格列酮能够改善兔二次打击MODS模型的器官功能.“,”[Objective]To investigate the PPAR-γ,NF-κB,TNF-α, IL-10 expression,blood gas and the biochemical changes in double-hit rabbit animal model of multiple organ dysfunction syndrome (MODS).The effects of rosiglitazone and GW9662 were evaluated.[Methods] In this study, we prepared double-hit MODS rabbit model through fluid resuscitation after hemorrhagic shock and LPS stimulation.We used PPAR-γ agonist rosiglitazone and PPAR-γ antagonist GW9662 to regulate the expression of PPAR-γ.We detected the expression of PPAR-γ, NF-κB, TNF-α, and IL-10 in liver 24 hour after the double-hit MODS model was established.Biochemical and blood gas analysis at the same time were detected as well.[Results]The expressions of PPARγ mRNA in T-H group and D-H-S group were significant lower than that in sham group (P<0.05).In the D-H group, the expression of PPARγ mRNA with rosiglitazone application was higher than that in control group (P<0.05) whilethe expression of PPARγ mRNA with GW9662 application was significant lower than that in rosiglitazone-treated group(P<0.05).In the T-H group, the expressions of NF-κB and TNF-α increased significantly (P0.05).In D-H-S group, the expressions of NF-κB and TNF-α increased significantly as compared to T-H group (P<0.05).The expressions of IL-10 in D-H-S group increased significantly as compared to sham group (P0.05).In D-H-R group, the expressions of NF-κB and TNF-α decreased significantly compared with D-H-S group (P<0.05).The expressions of IL-10 increased significantly compared with D-H-S group (P<0.05).In D-H-G group, the expressions of NF-κB and TNF-α increased significantly compared with D-H-R group (P<0.05).The expressions of IL-10 decreased significantly compared with D-H-R group (P<0.05).In the T-H group, blood gas and biochemical parameters did not change significantly compared with sham group.In the D-H-S group, blood gas and biochemical parameters increased significantly compared with sham group and T-H group (P<0.05).In the D-H-R group, blood gas and biochemical parameters ameliorated significantly compared with D-H-S group (P<0.05).In the D-H-G group, blood gas and biochemical parameters deteriorated significantly compared with D-H-R group (P<0.05).[Conclusion]As the agonist of PPAR-γ, Rosiglitazone could improve the organ function in MODS rabbit induced by double-hit.