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目的 分析非小细胞肺癌 (non- small- cell lungcancer,NSCL C)病人肿瘤浸润性淋巴细胞 (tum or- infiltrat-ing lymphocytes,TIL)的 T细胞受体 (TCR) Vβ2 4个亚家族基因的克隆性增殖及分布情况。方法 肺腺癌病人 1例 ,术中取新鲜肺癌组织 ,肺癌组织经处理后分别与自身肿瘤细胞共同培养 3.5天和 7天。 10例健康人、T细胞株 Jurkat和 B细胞株 Raji作为对照。标本共 16份 ,提取 m RNA后经反转录多聚酶链反应 (RT- PCR) ,PCR产物进一步经基因扫描分析 Vβ2 4个亚家族基因的互补决定区 3(CDR3)以确定 T细胞克隆性。结果 NSCL C病人外周血 T细胞仅表达 16个Vβ亚家族 ,Vβ6和 Vβ17呈寡克隆。新鲜肺癌组织 TIL仅检测到 Vβ19表达 ,呈寡克隆 ;培养 7天后 ,检测 7个 Vβ亚家族表达 ,Vβ8和 Vβ16呈寡克隆。 10例正常人 T细胞表达所有 2 4个 Vβ亚家族且均呈多克隆性分布。结论 NSCL C病人 TIL中 ,TCR Vβ2 4个亚家族存在倾斜性分布 ,T细胞存在克隆性增殖。这可能是肿瘤相关抗原 (tumor associatedantigens,TAA)刺激引起的特异性 T细胞免疫反应。研究结果提示 ,构成经培养的 TIL 的主要 T细胞成分是克隆性增殖的表达有限几个 TCR Vβ亚家族的 T细胞 ,这些克隆性增殖 T细胞可能是 TIL 对自身肿瘤细胞具有?
Objective To analyze the cloning of TCR (TCR) Vβ2 4 subfamilies of tumor-infiltrat-ing lymphocytes (TIL) in patients with non-small cell lung cancer (NSCL C). Sexual proliferation and distribution. Methods One case of adenocarcinoma of the lung was treated with fresh lung cancer. After treatment, lung cancer tissues were incubated with their own tumor cells for 3.5 days and 7 days, respectively. Ten healthy people, T cell strain Jurkat and B cell strain Raji were used as controls. A total of 16 specimens were obtained, and m RNA was extracted and subjected to reverse transcription-polymerase chain reaction (RT-PCR). The PCR products were further subjected to gene scanning to analyze the complementarity determining region 3 (CDR3) of Vβ2 4 subfamily genes to determine the clonality of T cells. Results In peripheral blood T cells of NSCL C patients, only 16 Vβ subfamilies were expressed, and Vβ6 and Vβ17 were oligoclonal. TIL from fresh lung cancer tissues only detected Vβ19 expression and showed oligoclonal. After 7 days of culture, 7 Vβ subfamily genes were detected. Vβ8 and Vβ16 were oligoclonal. Ten normal human T cells expressed all 24 Vβ subfamily and all showed a polyclonal distribution. Conclusion In TIL of patients with NSCL C, TCR Vβ2 4 subfamilies have a skewed distribution, and T cells have clonal proliferation. This may be a specific T cell immune response induced by tumor associated antigens (TAA) stimulation. The results suggest that the main T cell component that constitutes the cultured TIL is a clonally expanded T cell that expresses a limited number of TCR Vβ subfamily. These clonally proliferating T cells may be TILs that have autologous tumor cells?