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目的 研究pcDNA3 1·PTH治疗甲状旁腺功能低下的治疗效果及最佳剂量。方法 手术切除家兔甲状旁腺 ,建立甲状旁腺功能低下症模型。将已构建的人甲状旁腺激素基因pcDNA3 1·PTH以 50 μg/kg、1 50 μg/kg、2 50 μg/kg剂量注射模型家兔的骨骼肌内 ,测定注射后不同时间的血钙及血PTH。结果 注射pcDNA3 1·PTH后 2 4h模型家兔的血钙及PTH升高 ,肌注 1 50 μg/kg组的模型兔 ,血钙和PTH值在 7d时达正常水平 ,各为 :2 83± 0 0 2 (mmol/L)、1 1 30 3± 0 0 2 5(pg/ml)。肌注 2 50 μg/kg组的模型兔 ,血钙和PTH值在 7d时达正常水平 ,各为 :2 81± 0 0 7(mmol/L)、1 2 0 0 1± 0 0 0 8(pg/ml)。并均持续 2个月时间。结论 人甲状旁腺激素基因治疗甲状旁腺功能低下是有效的 ,且以 1 50 μg /kg和 2 50 μg/kg为最佳剂量
Objective To study the therapeutic effect and optimal dosage of pcDNA3 1 · PTH for treating hypoparathyroidism. Methods Surgical removal of rabbit parathyroid glands, the establishment of hypoparathyroidism model. The constructed human parathyroid hormone gene pcDNA3 1 · PTH was injected into the skeletal muscle of rabbits at a dose of 50 μg / kg, 150 μg / kg and 250 μg / kg respectively, and the levels of serum calcium and Blood PTH. Results The levels of serum calcium and PTH were increased in the model rabbits injected with pcDNA3 1 · PTH at 24 h, and the levels of serum calcium and PTH in the model rabbits with intramuscular injection of 50 μg / kg reached the normal levels at 7 days (2 83 ± 0 0 2 (mmol / L), 1 1 30 3 ± 0 0 2 5 (pg / ml). Intramuscular injection of 50 μg / kg group of rabbits, serum calcium and PTH values at 7d reached the normal levels, respectively: 2 81 ± 0 0 7 (mmol / L), 12 0 0 ± 0 0 0 8 ( pg / ml). And all last 2 months. Conclusion Human parathyroid hormone gene therapy for hypoparathyroidism is effective, and the best dose of 150 μg / kg and 50 μg / kg