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近年来,由于人口统计学及社会的需要,人类性比的遗传学研究,已引起多方关注。而由于人类希望更多地影响后代性别形式,确定原始性比,即受孕时胚胎的性比率,更显重要。作者对214例孕8周以内人工流产胚胎的性别作了鉴定。分别研究<6周及6～8周的胚胎性比。同时对172例<8周及551例14～26周的自然流产胚胎性比以及19,842例新生儿的性比(第二性比)进行了分析。方法:取胚胎上皮,不经培养制成涂片。一部分用甲醇及醋酸混合液固定后用芥子奎吖因染色,查Y染色体。至少在5～6个细胞内发现有Y染色体时,胚胎性别决定为男性。另一部分涂片用乙醚酒精混合液固定,查X染色体。当200个细胞内的阳性率>15时,胚胎性别决定为女性。
In recent years, due to the needs of demography and society, the genetic studies of human sex ratio have drawn much attention. However, since mankind wants to influence the gender form of the offspring more, it is more important to determine the original ratio, that is, the sex ratio of the embryo at the time of conception. The author of 214 cases of pregnancy within 8 weeks of abortion embryos were identified sex. The embryo ratios <6 weeks and 6-8 weeks were studied separately. At the same time, 172 cases <8 weeks and 551 cases of 14-26 weeks of spontaneous abortion embryo sex ratio and 19,842 cases of newborns sex ratio (second sex ratio) were analyzed. Methods: Embryonic epithelium was taken without smear. Part of methanol and acetic acid mixture was fixed with mustard quinacrine staining, check the Y chromosome. At least 5 to 6 cells found Y chromosome, the embryo sex decision for men. Another part of the smear fixed with ether mixture of alcohol, check the X chromosome. When the positive rate was> 15 in 200 cells, the sex of the embryo was determined to be female.