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目的:观察重组别藻蓝蛋白(rAPC)对接种H22肝癌细胞小鼠的抑瘤活性及其免疫作用。方法:昆明小鼠随机分为5组(10只/组),即模型组、rAPC低、中、高剂量组(25,50,100mg/kg·d)、环磷酰胺组(CY对照组)。建立小鼠肝癌H22移植瘤模型,次日除模型组外,其余4组分别以不同剂量的rAPC、CY灌胃,于15d后处死,完整剥离出肿瘤、胸腺、脾脏,准确称重,计算抑瘤率、胸腺指数和脾指数,并采用放免法(RIA)测定血清中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的水平。结果:rAPC低剂量组小鼠H22肿瘤质量增长较模型组缓慢(P<0.05),中、高剂量组较模型组显著缓慢(P<0.01),抑瘤率分别为25.2%、36.7%、43.1%;rAPC各剂量组能升高胸腺指数、脾指数和血清中细胞因子IL-6、TNF-α的水平。结论:rAPC可有效抑制H22肝癌的生长,促进小鼠胸腺和脾脏的生长发育,提高小鼠的免疫功能,从而抑制肿瘤的生长。
Objective: To observe the anti-tumor activity and immune effects of recombinant allophycocyanin (rAPC) on mice inoculated with H22 hepatoma cells. Methods: Kunming mice were randomly divided into 5 groups (10/group): model group, low, medium, and high doses of rAPC (25, 50, 100 mg/kg·d), and cyclophosphamide (CY control). Mouse hepatoma H22 xenograft tumor model was established. In the next day, except for the model group, the other 4 groups were administered with different doses of rAPC and CY, respectively, and were sacrificed 15 days later. The tumors, thymus, and spleen were completely detached and accurately weighed. The tumor rate, thymus index, and spleen index were measured by radioimmunoassay (RIA) to determine the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum. Results: Compared with the model group, the tumor growth of hA22 mice in the rAPC low-dose group was slower (P<0.05), and was significantly slower in the middle- and high-dose groups than in the model group (P<0.01). The tumor inhibition rates were 25.2%, 36.7%, and 43.1 respectively. %; rAPC dose group can increase thymus index, spleen index and serum IL-6, TNF-α levels. Conclusion: rAPC can effectively inhibit the growth of H22 liver cancer, promote the growth and development of mice thymus and spleen, and increase the immune function of mice, thus inhibiting the growth of tumors.