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为了获得生产用廉价型灵芝发酵培养基,采用中心组合旋转设计法和响应曲面法对低成本培养基组分进行了优化。优化的四个组分为玉米粉(x1)、麸皮粉(x2)、豆饼粉(x3)和蔗糖(x4)。结果表明,灵芝菌体发酵和多糖发酵的培养基预测模型分别为:Y1=15.1–0.31x1–0.34x2+0.36x3–0.44x4–1.26x12–1.98x22–0.85x32–1.15x42–0.59x2x3和Y2=2.0–0.08x1–0.08x2+0.04x3–0.09x4–1.13x12–0.33x22–0.08x32–0.16x42–0.16x2x3–0.10x1x4。从中获得菌体发酵的最优配方为:玉米粉19.7g/L,麸皮粉11.3g/L,豆饼粉6.3g/L,蔗糖19.5g/L;多糖发酵的最优配方为:玉米粉19.6g/L,麸皮粉11.0g/L,豆饼粉6.7g/L,蔗糖19.1g/L。150L发酵罐中试放大结果表明,灵芝菌体的产量为16.92g/L,多糖产量为1.86g/L。所得培养基为灵芝产品的高效低成本生产提供了基础。
In order to obtain a low-cost Ganoderma lucidum fermentation medium for production, the low-cost medium components were optimized using a central combination of rotational design and response surface methodology. The optimized four components are corn meal (x1), bran meal (x2), soybean meal (x3) and sucrose (x4). The results showed that the prediction models of the medium of Ganoderma lucidum and polysaccharide fermentation were as follows: Y1 = 15.1-0.31x1-0.34x2 + 0.36x3-0.44x4-1.26x12-1.98x22-0.85x32-1.15x42-0.59x2x3 and Y2 = 2.0-0.08x1-0.08x2 + 0.04x3-0.09x4-1.13x12-0.33x22-0.08x32-0.16x42-0.16x2x3-0.10x1x4. The optimal formula of microbial fermentation was 19.7g / L corn meal, 11.3g / L bran meal, 6.3g / L soybean meal and 19.5g / L sucrose. The optimal formula of polysaccharide fermentation was 19.6g g / L, bran powder 11.0g / L, soybean meal 6.7g / L, sucrose 19.1g / L. 150L fermentor pilot enlargement results show that the production of Ganoderma lucidum cell was 16.92g / L, polysaccharide yield of 1.86g / L. The resulting medium provides a basis for efficient and low-cost production of Ganoderma lucidum products.