论文部分内容阅读
目的探讨鞣花酸对四氧嘧啶糖尿病小鼠的影响。方法 40只雄性昆明小鼠随机分为正常对照(Con)组10只和四氧嘧啶处理组30只。四氧嘧啶处理组腹腔注射2%四氧嘧啶,将造模成功的21只小鼠分为模型(Model)亚组及鞣花酸低、高剂量亚组,各7只。鞣花酸低、高剂量亚组分别以160mg/kg、320 mg/kg鞣花酸连续灌胃21 d。21 d后处死动物,取肝脏和血清,测定血清ALT,AST活性,肝脏SOD活性及MDA含量等。结果与Model亚组比较,鞣花酸高剂量亚组血糖[(29.12±7.99)vs(19.30±8.08)mmol/L],血清ALT水平[(143.15±57.16)vs(65.38±33.61)U/L]、AST水平[(156.60±39.70)vs(101.11±44.16)U/L]及MDA含量[(2.81±0.81)vs(1.62±0.41)mmol/g]均下降(P<0.05),肝脏SOD活性升高[(74.23±18.82)vs(109.94±23.33)U/mg]。结论鞣花酸有降低四氧嘧啶糖尿病小鼠血糖的作用,以320 mg/kg剂量最为明显。
Objective To investigate the effects of ellagic acid on alloxan diabetic mice. Methods Forty male Kunming mice were randomly divided into normal control (Con) group (n = 10) and alloxan-treated group (n = 30). Alloxan-treated groups were injected intraperitoneally with 2% alloxan. The 21 successful mice were divided into model subgroup and low and high dose ellagic acid subgroups, each with 7 mice. Ellagic acid low and high dose subgroup were 160mg / kg, 320 mg / kg ellagic acid continuous gavage for 21 days. After 21 days, the animals were sacrificed and the liver and serum were taken for measurement of serum ALT, AST activity, liver SOD activity and MDA content. Results Compared with the model subgroup, high-dose ellagic acid subgroups (29.12 ± 7.99 vs 19.30 ± 8.08 mmol / L) and serum ALT levels (143.15 ± 57.16 vs 65.38 ± 33.61 U / L (P <0.05). The level of AST in the liver (156.60 ± 39.70 vs 101.11 ± 44.16 U / L) and the content of MDA (2.81 ± 0.81 vs 1.62 ± 0.41 mmol / g) Increased [(74.23 ± 18.82) vs (109.94 ± 23.33) U / mg]. Conclusion Ellagic acid has the effect of reducing the blood sugar of alloxan diabetic mice, the most obvious is 320 mg / kg dose.