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目的:研究小儿清瘟解热颗粒对小鼠免疫功能的影响及体内、外抗菌作用。方法:取Balb/C小鼠70只,将小鼠按体重分为正常组、模型组、玉屏风颗粒组(2.8 g·kg-1),胸腺肽组(9.2 mg·kg-1),小儿清瘟解热颗粒低、中、高剂量组(10.92,21.84,43.68 g·kg-1),每组10只,除正常组外,其余各组通过小鼠ip环磷酰胺构建免疫缺陷小鼠模型,观察小儿清瘟解热颗粒对T,B淋巴细胞增殖的影响及对碳廓清能力,血清、脾细胞溶血素含量的影响;取ICR小鼠只,按体重等级随机分为7组,分别为模型组,阿莫西林组(370 mg·kg-1),小儿感冒宁合剂组(8.25 m L·kg-1),小儿清瘟解热颗粒低、中、高剂量组(3.2,6.4,12.8 g·kg-1),每组20~22只,采用小鼠腹腔注射金黄色葡萄球菌构建体内细菌感染模型,观察动物死亡数、死亡率,药物的保护率,动物的平均存活天数及生命延长率;在体外采用试管法观察药物对金黄色葡萄球菌、表皮葡萄球菌、白色葡萄球菌、大肠埃希菌、绿脓杆菌、乙性溶血性链菌、白色念珠菌、淋球菌及变形杆菌的最低抑菌浓度(MIC)。结果:小儿清瘟解热颗粒低、中剂量均可显著提高脂多糖(LPS),刀豆球蛋白A(Con A)诱导的淋巴细胞增殖;对于金黄色葡萄球菌体内感染模型,小儿清瘟解热颗粒可使动物死亡率明显降低,动物存活天数明显延长(与模型组比较P<0.05,P<0.01);同时,小儿清瘟解热颗粒体外对金黄色葡萄球菌、表皮葡萄球菌、白色葡萄球菌、大肠埃希菌、绿脓杆菌、乙性溶血性链菌、白色念珠菌及变形杆菌生长均有不同程度的抑制作用。结论:小儿清瘟解热颗粒对免疫低下小鼠的免疫功能具有一定的提高作用,在体内对金黄色葡萄球菌感染致小鼠死亡具有明显的保护作用,在体外对多种细菌的生长具有不同程度的抑制作用。
OBJECTIVE: To study the effect of Qingrejie Jieyang Granule on immune function in mice and the antibacterial effect in vitro and in vivo. Methods: Seventy Balb / C mice were divided into normal group, model group, Yupingfeng granule group (2.8 g · kg-1), thymosin group (9.2 mg · kg-1) The mice in low, medium and high dose group (10.92,21.84,43.68 g · kg-1) were treated with puerarin-heat granules, and the mice in each group were immunosuppressed , To observe the effect of Qingrejiemiaoli granules on the proliferation of T and B lymphocytes and their effects on the carbon clearance ability, serum and splenocyte hemolysin content. The ICR mice were randomly divided into 7 groups The model group, the amoxicillin group (370 mg · kg-1), the pediatric flu-sen mixture group (8.25 m L · kg-1), children with Qingrejieganji particles low, medium and high dose groups (3.2,6.4,12.8 g · kg-1, 20 ~ 22 in each group. The model of bacterial infection was established by intraperitoneal injection of Staphylococcus aureus in mice. The number of deaths, the mortality rate, the protection rate of drugs, the average survival days of animals and the life prolongation were observed In vitro vitro test using drugs on Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus bovis, white Candida, Neisseria gonorrhoeae and Proteus minimum inhibitory concentration (MIC). Results: The low and medium doses of Qingrejiedu granules in children could significantly increase the proliferation of lymphocytes induced by lipopolysaccharide (LPS) and concanavalin A (Con A). For the in vivo infection model of Staphylococcus aureus, Thermal particles can significantly reduce the mortality rate of animals, animals significantly longer survival days (compared with the model group P <0.05, P <0.01); the same time, children with Qingrejie Ke particles Staphylococcus aureus, Staphylococcus epidermidis, white grapes Cocci, Escherichia coli, Pseudomonas aeruginosa, streptococcus thermophilus, Candida albicans and Proteus growth have different degrees of inhibition. CONCLUSION: Pediatric Qingwen Jieji granules can improve the immune function of mice with immunosuppression, protect the mice from death caused by Staphylococcus aureus in vivo, and have different effects on the growth of many bacteria in vitro Degree of inhibition.