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用7个我国当前流行的条锈菌生理小种评价中梁21的苗期条锈抗性,结果表明该品种对我国优势流行小种具有良好的抗性。采用CYR30小种对中梁21与铭贤169杂交的F1、BC1、F2及F3代群体进行遗传分析,并利用SSR分子标记进行遗传作图,发现中梁21对CYR30的抗性由1个显性基因控制,暂命名为Yrzhong21。该基因与位于小麦5AL染色体上的10个SSR位点Xgwm186、Xbarc165、Xwmc795、Xbarc40、Xgwm156、Xgwm617、Xwmc415、Xbarc151、Xwmc338和Xgwm666连锁,其中最近的侧翼位点为Xgwm186和Xbarc165,其遗传距离分别是7.5cM和2.7cM。系谱分析结合分子标记结果表明,该基因可能来自Ciemenp。与已定位于5A染色体上的抗条锈病基因的比较表明,Yrzhong21可能是一个抗条锈病的新基因。用标记Xgwm186和Xbarc165检测中梁系列品种,其中仅17%扩增到与中梁21相同的位点,表明该基因在抗条锈病育种中可能有很大的应用潜力。
The resistance of stripe rust of Zhongliang 21 to seedling stage was evaluated by 7 current race strains of stripe rust in China. The results showed that this variety had good resistance to the dominant race of our country. Genetic analysis of F1, BC1, F2 and F3 populations of cross between Zhongliang 21 and Mingxian 169 by CYR30 races and genetic mapping using SSR markers showed that the resistance of Zhongliang 21 to CYR30 was marked by one significant Sexual gene control, tentatively named Yrzhong21. The gene was linked to 10 SSR loci Xgwm186, Xbarc165, Xwmc795, Xbarc40, Xgwm156, Xgwm617, Xwmc415, Xbarc151, Xwmc338 and Xgwm666 located on the 5AL chromosome of wheat with the closest flanking sites being Xgwm186 and Xbarc165, respectively Is 7.5 cM and 2.7 cM. Genealogy analysis combined with molecular marker results showed that the gene may come from Ciemenp. Comparison with the stripe rust resistance genes mapped on chromosome 5A indicated that Yrzhong21 may be a new gene resistant to stripe rust. Detection of the series of Zhongliang lines with markers Xgwm186 and Xbarc165, of which only 17% amplified to the same locus as Zhongliang 21, indicated that the gene may have great potential for application in the breeding of resistance to stripe rust.