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目的:探讨N-乙酰基半胱氨酸(NAC)对口腔常用水门汀:磷酸锌水门汀(Zpc)、玻璃离子水门汀(FujiⅠ)、树脂改良型玻璃离子水门汀(Fuji plus)、树脂水门汀(G-cem)体外细胞毒性的影响。方法:四种水门汀Zpc、FujiⅠ、Fuji plus、G-cem固化制取标准模型后用培养液制取浸提液,每组各加入不同浓度NAC(0 m M;1 m M;5 m M;10 m M;15m M),再分别与3T3细胞系(小鼠胚胎成纤维细胞)共培养,采用四唑盐显色(MTT)比色分析法,比较不同水门汀加入不同浓度NAC后细胞毒性的变化。结果:四种水门汀Zpc、FujiⅠ、Fuji plus、G-cem具有不同程度的细胞毒性,表现为细胞相对增值率(RGR)的降低,其RGR均值分别为94.72%、80.89%、31.44%、3.89%。而加入不同浓度NAC后各水门汀表现不一:FujiⅠ和Fuji Plus加入1 m M、5 m M NAC时RGR有增高趋势,但无统计学差异,反而在加入15 m M NAC时出现RGR的降低(P<0.05);G-cem和Zpc加入NAC后都相应的降低水门汀细胞毒性,提高细胞相对增殖率,其中5m M和10 m M出现峰值(P<0.05)。结论:不同水门汀其生物相容性差别较大,临床在选择水门汀时应该注意其毒性的影响;加入NAC后可以改善其生物相容性,但是NAC具有浓度依赖性,要注意浓度的选择。NAC减毒机理有待进一步研究。
OBJECTIVE: To investigate the effect of NAC on the oral cavity commonly used cements: zinc phosphate cement (Zpc), glass ionomer (Fuji I), resin modified glass ionomer (Fuji plus), resin cements ) In vitro cytotoxic effects. Methods: Four kinds of cements, Zpc, FujiⅠ, Fuji plus and G-cem, were hardened to prepare a standard model. The extracts were prepared from the culture medium and each group was given different concentrations of NAC (0 m M 1 m M 5 m M; 10 m M and 15 m M respectively) were co-cultured with 3T3 cell line (mouse embryonic fibroblasts). MTT colorimetric assay was used to compare cytotoxicity of different cement with different concentrations of NAC Variety. Results: Four kinds of cements, Zpc, FujiⅠ, Fuji plus and G-cem, showed different degrees of cytotoxicity. The RGR values were 94.72%, 80.89%, 31.44% and 3.89% . However, the addition of different concentrations of NAC did not show the same trend: RGR increased with addition of Fuji I and Fuji Plus at 1 m M and 5 m N NAC, but there was no statistical difference. RGR decreased at 15 m N NAC P <0.05). After adding NAC, both G-cem and Zpc decreased the cytotoxicity of cements and increased the relative proliferation rate of cells, with 5m M and 10m M peaked (P <0.05). CONCLUSIONS: Different cements have different biocompatibility. Clinics should pay attention to their toxicity when selecting cements. Adding NAC can improve its biocompatibility, but NAC is concentration-dependent, and attention should be paid to the choice of concentration. NAC attenuated mechanism needs further study.