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本研究在体外培养的条件下研究了BDNF、SDNF、GM1对大鼠胚腹侧中脑多巴胺神经元生长发育的影响。取孕15d鼠胚腹侧中脑细胞悬液将之接种于24孔培养板中进行培养。实验分4组；对照组及分别向培养液中加入BDNF、SDNF、GM1等的三个实验组。培养7d后取出。用酪氨酸羟化酶免疫组织化学ABC法观察和比较了多巴胺神经元的生长状态。发现加入BDNF、SDNF、GM1的培养孔内的酪氨酸羟化酶阳性神经元明显增多，细胞突起的长度增加且数量增多，与单纯培养组相比均有显著性差异，但三个实验组之间无显著性差异。研究结果表明，BDNF、SDNF、GM1可促进体外培养的鼠胚腹侧中脑多巴胺神经元的存活及突起的延伸。
In this study, the effects of BDNF, SDNF and GM1 on the growth and development of embryonic ventral midbrain dopamine neurons were studied in vitro. The embryonic ventral midbrain cell suspension was inoculated into 24-well culture plates for culture on the 15th day. The experiment was divided into 4 groups; the control group and three experimental groups which were added BDNF, SDNF, GM1 respectively to the culture medium. Removed after cultivation for 7 days. Tyrosine hydroxylase immunohistochemical ABC method to observe and compare the growth of dopamine neurons. The results showed that tyrosine hydroxylase positive neurons in BDNF, SDNF and GM1 culture cells were significantly increased, and the length of the cells increased and the number increased, which was significantly different from that of the pure culture group, but the three experimental groups No significant difference between. The results showed that BDNF, SDNF and GM1 could promote the survival and protuberance of dopaminergic neurons cultured in vitro.