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目的 研究一氧化氮合酶（ＮＯＳ）抑制剂在局灶性脑缺血再灌流过程中对神经细胞的作用机理，特别是ＮＯＳ抑制剂（ＮＮＬＡ）与细胞凋亡的关系。方法利用末端标记法，测定ＮＮＬＡ干预后局灶性脑缺血鼠脑组织中神经细胞的凋亡情况。结果 大、小剂量的ＮＯＳ抑制剂分别具有增加或减少阳性凋亡细胞出现率的作用。结论给予不同剂量的ＮＯＳ抑制剂与单纯缺血再灌流相比，对脑神经细胞的凋亡出现率有不同的影响。
Objective To study the mechanism of action of nitric oxide synthase (NOS) inhibitor on nerve cells during focal cerebral ischemia and reperfusion, especially the relationship between NOS inhibitor (NNLA) and apoptosis. Methods The apoptosis of neural cells in brain tissue of focal cerebral ischemia rats after NNLA intervention was detected by end-labeling method. Results Large and small doses of NOS inhibitors have the role of increasing or decreasing the incidence of positive apoptotic cells, respectively. Conclusion Compared with ischemia / reperfusion alone, NOS inhibitor at different dosages has different effects on the incidence of apoptosis in brain neurons.