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目的了解目前市售婴幼儿配方奶粉、谷基辅助食品样品中蜡样芽胞杆菌的污染及其毒素基因的携带情况。方法采集石家庄市23个区县市售婴幼儿配方粉、谷基辅助食品共399份,依照国家标准GB/T 4789.14—2003《食品卫生微生物学检验蜡样芽胞杆菌检验》和《食源性致病菌监测工作手册》进行蜡样芽胞杆菌检测并计数,应用荧光PCR方法检测蜡样分离株的溶血素基因和非溶血素基因。结果 399份样品中蜡样芽胞杆菌检出85份,其中婴幼儿配方奶粉检出36份,检出率22.8%(36/158);谷基辅助食品检出49份,检出率20.3%(49/241)。85份阳性样品中有48份呈溶血素基因阳性,检出率56.5%,非溶血素基因均为阴性。结论婴幼儿食品中蜡样芽胞杆菌的污染较严重,存在潜在的食品风险。分析结果可为婴幼儿食品卫生学检验标准及监督管理等方面提供参考。
Objective To understand the contamination of Bacillus cereus and its toxin gene in the commercially available infant formula and cereal-based food samples. Methods A total of 399 infant formula powder and cereal-based auxiliary food were collected from 23 districts and counties in Shijiazhuang. According to the national standard GB / T 4789.14-2003, “Microbiological examination of food hygiene and Bacillus cereus” and “ Bacteria monitoring manual ”for Bacillus cereus detection and counting, fluorescence PCR method was used to detect waxy isolate hemolysin gene and non-hemolysin gene. RESULTS: Bacillus cereus was detected in 399 samples, of which 36 samples were detected by infant formula, the detection rate was 22.8% (36/158); 49 basal-based auxiliary products were detected with the detection rate of 20.3% 49/241). 48 of 85 positive samples were positive for hemolysin gene, the detection rate was 56.5%, non-hemolysin genes were negative. Conclusion The contamination of Bacillus cereus in infants and young children food is more serious and there is potential food risk. The results of the analysis can provide reference for infant food hygiene inspection standards and supervision and management.