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目的 :探讨早孕绒毛组织中IGF IImRNA表达水平的变化与胎盘、胚胎早期发育的关系。方法 :采用逆转录聚合酶链反应 (RT PCR)检测 2 0例正常早孕妇女 (正常对照组 )、15例自然流产妇女 (自然流产组 )及 2 0例药物流产妇女 (药物流产组 )绒毛组织中IGF IImRNA的表达 ,同时用PAS染色法分别观测其绒毛组织中糖原储存水平的变化 ,并进行半定量分析和相关性分析。结果 :(1)IGF IImRNA在自然流产组、药物流产组绒毛组织中的表达水平分别为 1.19± 0 .6 7、1.4 2± 0 .6 4,明显低于正常早孕组的 2 .2 2± 0 .95 ,差异均具有显著性 (P <0 .0 5 ) ,药物流产组与自然流产组差异无显著性 (P >0 .0 5 ) ;(2 ) 3组绒毛组织中糖原灰度值分别为 10 6 .0 4± 12 .6 0、82 .85± 2 2 .79、85 .94± 16 .15 ,自然流产组、药物流产组与正常对照组相比差异有显著性 (P <0 .0 5 ) ;(3)绒毛组织中IGF IImRNA的表达强度与其糖原储存水平呈正相关 (P <0 .0 5 )。结论 :推测早孕绒毛组织IGF IImR NA的适量表达与胎盘、胚胎发育有关 ,IGF对胎盘糖原合成的调节可能是其重要作用机制之一。
Objective: To investigate the relationship between the changes of IGF II mRNA expression in early villus tissues and the early development of placenta and embryo. Methods: Reverse transcription polymerase chain reaction (RT-PCR) was used to detect 20 normal pregnant women (normal control group), 15 spontaneous abortion women (spontaneous abortion group) and 20 abortion women (abortion group) IGF IImRNA expression in the same time, PAS staining were observed in the villus tissue glycogen storage changes, and semi-quantitative analysis and correlation analysis. Results: (1) The expression levels of IGF II mRNA in the spontaneous abortion group and the medical abortion group were 1.19 ± 0.67, 1.42 ± 0.64, respectively, which were significantly lower than those in the normal pregnancy group 0.95, the difference was significant (P <0.05), no significant difference between drug abortion group and spontaneous abortion group (P> 0.05); (2) The glycogen gray level The values were 10 6 .0 4 ± 12 .6 0,82 .85 ± 2 2 .79,85 .94 ± 16 .15 respectively. There was significant difference between spontaneous abortion group and medical abortion group and normal control group (P <0.05). (3) The expression of IGF II mRNA in villus tissues was positively correlated with the glycogen storage (P <0.05). Conclusion: It is speculated that the expression of IGF IImR NA in early pregnancy villi is related to placenta and embryonic development. The regulation of IGF on placental glycogen synthesis may be one of the important mechanisms.