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本试验以五个籼稻品种为材料,研究成熟种子经200Gy~(137)Csγ射线、2mM NaN_3单独和复合处理后的体细胞培养反应。试验结果表明,γ射线和NaN_3单独处理降低种子出愈率,愈伤组织生长率和分化率,γ射线+NaN_3复合处理明显降低种子出愈率、初代培养愈伤组织生长率和分化率,其处理效应高于单独处理效应。但第2、3次培养时,γ射线处理效应大于γ射线+NaN_3复合处理效应。根据本试验结果。认为在以水稻成熟种胚作外植体时,培养前采用200Gy~(137)Csγ射线+2mM NaN_3进行复合处理是可行的。
In this study, five indica rice varieties were used as materials to study the somatic cell culture response of mature seeds treated with 200 Gy ~ (137) Csγ ray and 2 mM NaN_3 alone or in combination. The results showed that γ ray and NaN_3 alone could reduce the rate of seed emergence, the rate of callus growth and differentiation, and the combination of γ-ray and NaN_3 significantly reduced the rate of seed emergence, the rate of callus growth and the differentiation rate of primary culture The treatment effect is higher than the individual treatment effect. However, in the second and third cultivation, the γ-ray treatment effect was greater than the γ-ray + NaN_3 compound treatment effect. According to the test results. It is considered that it is feasible to use 200Gy ~ (137) Csγ ray + 2mM NaN_3 for compound treatment before maturation of rice as explants.