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疟疾是一种严重危害人类健康和生命的疾病,我国输入性疟疾呈上升趋势,近年来国内外应用荧光定量PCR技术进行疟疾的快速检测和分型,在低密度疟原虫感染水平的检测更为敏感。荧光探针法是利用探针与靶序列特异杂交来指示扩增产物的增加,特异性高,但试剂价格昂贵,而且对样本处理有特殊要求。荧光染料法是利用它结合到双链DNA发射荧光信号指示扩增产物的增加,简便易行,相对便宜,但是不能用于多重检测。随着荧光定量PCR技术的不断发展,在标本处理、标记技术等领域有了新的进展,并在疟原虫耐药性监测方面有广泛应用。
Malaria is a serious disease that endangers human health and life. The incidence of imported malaria in our country is on the rise. In recent years, the rapid detection and typing of malaria using fluorescence quantitative PCR technology at home and abroad has made the detection of malaria infection at low density more sensitive. The fluorescent probe method utilizes the specific hybridization of the probe and the target sequence to indicate the increase of the amplification product, the high specificity, but the reagent is expensive, and the sample processing has special requirements. The fluorescent dye method utilizes the fact that it binds to double-stranded DNA and emits a fluorescent signal to indicate the increase of the amplification product, which is easy, relatively cheap, but can not be used for multiple detection. With the continuous development of fluorescence quantitative PCR technology, new progress has been made in such fields as specimen handling and labeling technology, and it has been widely used in the monitoring of Plasmodium resistance.