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目的对白首乌C21甾苷元告达庭甾苷元和开德甾苷元(Ⅱ)实现C11α-羟基化,要求副产物少,收率高。方法采用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法,羟化反应中控制值pH在5.0～6.0,于30℃温度下振荡培养70h。结果产物经IR、MS、NMR分析确认是C11α-羟基化了的告达庭甾苷元(Ⅲ)和开德甾苷元(Ⅲ),副产物少,收率达70.1%。结论用黑根霉(Rhizopus nigricans)和犁头霉(Absidia sp.)两种微生物在水-正丁醇双相体系中同步转化的方法是实现C11α-羟基化的有效方法。
Objective To achieve the C11α-hydroxylation of the C21 glycosides of P. bracteatum by using the C21 glycosides and the Kaideng glycosides (II). This method requires less by-products and high yields. Methods The method of simultaneous conversion of two microorganisms, Rhizopus nigricans and Absidia sp., was used in the water-n-butanol two-phase system. The value of the control value in the hydroxylation reaction was 5.0-6.0, and was 30. Incubate at 70 °C for 70 h. The resulting product was identified by IR, MS, and NMR as C11α-hydroxylated gartinone aglycon (III) and Kai Tak aglycone (III) with less by-products and a yield of 70.1%. Conclusion The simultaneous conversion of two microorganisms, Rhizopus nigricans and Absidia sp., in water-n-butanol biphasic system is an effective method to achieve C11α-hydroxylation.