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目的 探讨广谱组蛋白去乙酰化酶抑制剂(HDACI)伏立诺他(SAHA)对急性重症胰腺炎大鼠(SAP)早期炎症反应的影响.方法 采用牛磺酸钠胆胰管逆行注射诱发SAP大鼠模型,将36只大鼠随机分成三组,A组为假手术组、B组为SAP模型组、C组为SAP模型加SAHA组,观察比较各组大鼠胰腺组织病理损伤,血清淀粉酶、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,胰腺组织NF-κB和p38 MAPK蛋白表达.结果 与A组相比,B组胰腺组织病理损伤评分,血清淀粉酶、IL-6、TNF-α水平,胰腺组织NF-κB和p38 MAPK蛋白表达的水平均显著升高,差异有统计学意义(P<0.05).而C组与B组相比上述指标均有所下降,B组NF-κB蛋白表达水平为(1.23±0.12),C组为(0.56±0.13),差异有统计学意义(P<0.05);B组p38 MAPK蛋白表达水平为(1.07±0.18),C组为(0.71±0.11),差异有统计学意义(P<0.05).结论 SAHA能减轻SAP大鼠早期炎症反应,可为SAP的治疗提供新的方法.“,”Objective To explore the protective effect of the histone deacetylase inhibitor (HDACI) suberoylanilide hydroxamic acid(SAHA) on early inflammatory response in rats with severe acute pancreatitis(SAP). Methods The model of SAP was induced by the retrograde infusion of sodium taurocholic into the pancreatic duct. Based on random number table method ,36 rats were divided into three groups; group A was sham operation group ,B group was severe acute pancreatitis model group ,C group was severe acute pancreatitis model and SAHA group. Pancreatitis was evaluated by the pathological score. Serum amylase, IL-6 and tumor necrosis factor-α(TNF-α)levels were measured. Pancreatic NF-κB and p38 MAPK expression levels of three groups were detected. Results The rats in group B compared with group A ,the pancreas pathological score ,serum amylase IL-6 and TNF-αlevels, pancreatic NF-κB and p38 MAPK expression level were significantly increased ,while the above indexes of group C were decreased. Levels of pancreatic NF-κB expression were (1.23±0.12)for B group,and(0.56±0.13)for C group. Levels of pancreatic p38 MAPK expression were(1.07±0.18)in B group,and (0.71 ± 0.11) in C group. There were significant differences between the two groups(P<0.05). Conclusion SAHA could reduce early inflammation response in rats with SAP and provide a new therapeutic direction .