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目的 :探讨胃粘膜相关淋巴组织 (MALT)诊断与分类方法。方法 :对 14例MALT淋巴瘤进行免疫组化及病理形态定量测定研究。标本采用常规石蜡切片、HE染色及ABC法免组化染色 ,并应用HPIAS 10 0 0彩色图像分析系统对肿瘤细胞核的形态进行定量测定。结果 :14例胃MALT淋巴瘤中CCL细胞性 9例 ,小无裂、大无裂、T免疫母细胞性各 1例 ,CCL合并大细胞性淋巴瘤 2例。形态定量测定结果表明胃MALT淋巴瘤与对照组间 15项形态参数均有极显著性差异 (P <0 .0 0 1)。 5型肿瘤细胞核的15项形态学参数间亦大多数具有极显著性 (P <0 .0 0 1)或显著性差异 (P <0 .0 5)。尤其是细胞核的面积、周长、等效直径、面积体积、周长体积、长径、短径、实面积 8项指标随着肿瘤分类恶性程度的增高而增大 ,反映了肿瘤恶性程度的增加。结论 :形态测定的方法能够对胃MALT淋巴瘤作出准确的诊断 ,其对于MALT淋巴瘤提供了一种新的诊断方法 ,具有重要意义。通过形态测定对MALT淋巴瘤进行分类是可行的
Objective: To investigate the diagnosis and classification of gastric mucosa-associated lymphoid tissue (MALT). Methods: 14 cases of MALT lymphoma were studied by immunohistochemistry and pathological morphometry. Specimens were routinely paraffin-embedded, HE stained, and ABC-free histochemical staining. The HPIAS 100 color image analysis system was used to quantitatively determine the morphology of tumor nuclei. RESULTS: Among the 14 cases of gastric MALT lymphoma, 9 cases were CCL cell, 1 case was small and no crack, 1 had T-immunoblasts, and 2 cases had CCL combined with large cell lymphoma. Quantitative quantitative analysis showed that the 15 morphological parameters between gastric MALT lymphoma and control group were significantly different (P < 0.01). Most of the 15 morphological parameters of type 5 tumor nucleus were highly significant (P < 0.01) or significantly different (P <0.05). In particular, the area, perimeter, equivalent diameter, area volume, perimeter volume, long diameter, short diameter, and real area of the nuclear nucleus increased with the increase in malignancy of the tumor, reflecting an increase in the degree of malignancy of the tumor. . Conclusion: The morphometric method can accurately diagnose gastric MALT lymphoma, which provides a new diagnosis method for MALT lymphoma, which is of great significance. Classifying MALT lymphomas by morphometry is feasible