利用组织培养技术,特别是茎尖分生组织培养(简称茎尖培养),使感染病毒病的植物重新获得无病毒植物。现将此项技术介绍如下: 一、茎尖培养技术用茎尖培养获得无病毒植物的技术大致包括如下过程:首先要选择被病毒感染的合适亲本材料,切取长2～3厘米的茎端,去除外围的叶片及老叶。用常规方法进行表面消毒后,在无菌条件下于解剖 Tissue culture techniques, especially shoot apical meristem culture (referred to as shoot tip culture), allow virus-infected plants to regain virus-free plants. This technology is introduced as follows: First, shoot tip culture technology to obtain the virus-free plant tip culture techniques generally include the following: First, select the appropriate virus infected parent material, cut 2 to 3 cm long stem end, Remove peripheral leaves and old leaves. After the surface is disinfected by conventional methods, it is dissected under aseptic conditions