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目的 :探讨胃泌素对人结肠癌裸鼠移植瘤的影响。方法 :建立人结肠癌 SW480裸鼠移植瘤模型 ,实验动物分为 5肽胃泌素 (PG)组及对照组 ,观察 PG对移植瘤体积、重量的影响并进行流式细胞术分析。结果 :移植瘤的体积重量、细胞内 DNA、蛋白质含量、S期、G2 M期细胞数、增殖指数 PG组显著高于对照组 ,而 G0 / G1 期细胞数则显著低于对照组。结论 :胃泌素在体内具有促进人结肠癌 SW480细胞分裂和增殖的作用 ,为结肠癌患者施行内分泌治疗提供了实验依据
Objective: To investigate the effect of gastrin on human colon cancer xenografts in nude mice. METHODS: A xenograft tumor model of human colon carcinoma SW480 was established. Experimental animals were divided into 5-PG group and control group. The effect of PG on the volume and weight of the transplanted tumor was observed and analyzed by flow cytometry. RESULTS: The volume weight, intracellular DNA, protein content, tumor cell number, G2 M phase cell number and proliferation index of the transplanted tumor in the PG group were significantly higher than those in the control group, while the number of G0/G1 phase cells was significantly lower than that in the control group. Conclusion: Gastrin has the function of promoting the division and proliferation of human colon cancer SW480 cells in vivo, and provides experimental basis for the implementation of endocrine therapy in colon cancer patients.