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目的:了解台州市食源性金黄色葡萄球菌的污染状况及肠毒素基因的分布特征,为食品安全的风险评估和食源性致病菌的预防提供科学依据。方法:在2005年-2010年间按照监测网的工作要求对采集的各类食品样品进行金黄色葡萄球菌的定性检测,并对保存的菌株用多重PCR方法进行18种肠毒素基因(sea～seu)的检测分析。结果:380份食品样品中共有47份检出金黄色葡萄球菌,总检出率为12.4%,其中即食食品检出率最高为13.2%,生肉类为11.7%,豆制品为7.7%;保存的42株菌中共有22株菌检出12种肠毒素基因,基因携带率为52.4%,sea、seq、sei基因的检出率较高,分别为21.4%、16.7%、11.9%。结论:台州市市售食品中金黄色葡萄球菌的污染程度较高,肠毒素基因携带率较高,监测结果应引起食品监管部门的重视。
Objective: To understand the contamination of foodborne Staphylococcus aureus and the distribution of enterotoxin genes in Taizhou, and to provide a scientific basis for the risk assessment of food safety and the prevention of foodborne pathogens. Methods: The qualitative detection of Staphylococcus aureus was carried out on all kinds of food samples collected from 2005 to 2010 in accordance with the requirements of the monitoring network, and 18 kinds of enterotoxin genes (sea ~ seu) Test analysis. Results: Staphylococcus aureus was detected in 47 out of 380 food samples, with a total detection rate of 12.4%. Among them, the highest detection rate was 13.2% for instant food, 11.7% for raw meat and 7.7% for soy products. The results showed that 12 strains of enterotoxigenic genes were detected in 22 strains of bacteria. The gene carrying rate was 52.4%. The detection rates of sea, seq and sei genes were 21.4%, 16.7% and 11.9% respectively. Conclusion: The contamination level of Staphylococcus aureus in the foodstuffs available for sale in Taizhou City is high, and the carrier rate of enterotoxin gene is high. The monitoring results should be paid more attention by food regulatory authorities.