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本文应用~(125)IudR标记的人胃癌细胞SGC-7991作靶,研究了部分纯合的人脐血α干扰素及黄芪对人外周血NK细胞毒的影响,并对其促进NK活性的机理做了初步探讨。结果指出,人脐血干扰素和黄芪对NK细胞毒均有明显的促进作用,促进活性前者大于后者,但大剂量的干扰素或黄芪则可轻度抑制NK细胞毒。另外,干扰素和黄芪还可保护靶细胞抵抗NK细胞毒,其程度不及对NK活性的促进作用。干扰素和黄芪具有协同作用,两者联合处理效应细胞,可使NK细胞毒提高5～6倍。实验进一步表明,黄芪可诱生γ干扰素,黄芪对NK细胞毒的促进作用与其诱导的抗病毒活性相平行。干扰素对NK细胞毒的促进作用与细胞的大分子代谢有关,推测,干扰素除能直接迅速激活NK细胞毒外,还可诱导淋巴细胞产生其它因子,进一步扩大干扰素对NK细胞毒的激活效应。
In this study, ~(125)IudR-labeled human gastric cancer cells SGC-7991 were used as targets to study the effects of partially homozygous human umbilical cord blood interferon-alpha and astragalus membranaceus on human peripheral blood NK cytotoxicity, and their mechanism of promoting NK activity. Made a preliminary discussion. The results indicated that both human umbilical cord blood interferon and astragalus membranaceus could significantly promote NK cytotoxicity, and the former was more active than the latter, but large doses of interferon or astragalus could slightly inhibit NK cytotoxicity. In addition, interferon and astragalus can also protect target cells against NK cytotoxicity to a lesser degree than NK activity. Interferon and astragalus have a synergistic effect. The combination of the two to treat effector cells can increase NK cytotoxicity by 5-6 times. The experiment further shows that Astragalus can induce gamma interferon, and the promoting effect of Astragalus on NK cytotoxicity is parallel to its induced antiviral activity. The promotion effect of interferon on NK cytotoxicity is related to the macromolecular metabolism of cells. It is speculated that interferon can directly activate NK cytotoxicity, but also induce other factors of lymphocytes to further expand the activation of NK cytotoxicity by interferon. effect.