很多外源性化学物质对T淋巴细胞数会产生一定的影响。鉴别T、B淋巴细胞的方法很多,主是根据膜的特性如用玫瑰花形成试验、免疫萤光染色等方法,也可借助扫描电镜将T、B淋巴细胞区别开来,以上方法操作繁杂,必须具备一定的条件,因此在应用上受到一定限制。1975年,Mueller首先发现酸性α-醋酸萘酯酶(Acid α-naphthylacetate esterse,ANAE)能特异地标记小鼠T淋巴细胞,在光学显微镜下即可鉴别T、B淋巴细 Many exogenous chemicals have a certain impact on the number of T lymphocytes. Identification of T, B lymphocyte many ways, the main film is based on the characteristics such as the use of roses formation test, immunofluorescence staining and other methods, but also by scanning electron microscopy T, B lymphocytes to distinguish the above method of operation complicated, Must have certain conditions, so the application is subject to certain restrictions. In 1975, Mueller first discovered that Acid α-naphthylacetate esters (ANAE) can specifically label mouse T lymphocytes and identify T and B lymphocytes under light microscope