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用一对选自脊髓灰质炎病毒及一对由Ｈｙｙｐｉａ所设计的细小ＲＮＡ病毒核酸５’端非编码区的引物，逆转录并扩增脊髓灰质炎病毒及其它肠道病毒的核酸，从而把脊髓灰质炎病毒和其它非脊髓灰质炎肠道病毒进行鉴别。１０５份被血清中和试验定型为脊髓灰质炎病毒的标本，逆转录一聚合酶链反应（ＲＴ─ＰＣＲ）的结果亦定为脊髓灰质炎病毒，灵敏度为１ｍ％；１３３份被血清中和试验定为非脊髓灰质炎肠道病毒的标本，均被判定为非脊髓灰质炎病毒，特异度为１００％。该方法可用于脊髓灰质炎病毒的检测及脊髓灰质炎病毒与其它非脊髓灰质炎肠道病毒的鉴别。
Using a pair of primers selected from the group consisting of poliovirus and a pair of non-coding regions of the 5 ’end of the nucleic acid of picornavirus designed by Hyypia, reverse transcription and amplification of nucleic acids of poliovirus and other enteroviruses, The plague virus and other non-polio enteric viruses were identified. 105 were screened by the serum neutralization test as poliovirus samples, the result of reverse transcription-polymerase chain reaction (RT-PCR) was also determined as poliovirus, the sensitivity was 1m%; 133 were serum neutralization test Specimens of non-polio enterovirus were all classified as non-poliovirus with a specificity of 100%. This method can be used for the detection of poliovirus and the identification of poliovirus and other non-polio enteroviruses.