论文部分内容阅读
目的研究安徽省宁国市有螺无病地区钉螺与日本血吸虫的相容性,探索有螺无病地区形成的原因。方法采集宁国市有螺无病地区钉螺600只,随机分为2组,每组300只。在实验室条件下以本省日本血吸虫毛蚴进行钉螺群体感染,两组钉螺与毛蚴比例分别为1∶20和1∶40。以相同方法感染铜陵县的肋壳钉螺和泾县的光壳钉螺作为对照。感染后将钉螺置于室内22～24℃温度下饲养90d,观察各组钉螺感染率和死亡情况。结果在1∶20组和1∶40组中,宁国市有螺无病地区钉螺均未能被日本血吸虫成功感染,而铜陵县的肋壳钉螺和泾县的光壳钉螺均感染成功。至观察期结束时,无论是自然死亡率还是感染后死亡率,铜陵县的肋壳钉螺均显著低于宁国市有螺无病地区钉螺和泾县的光壳钉螺(均P<0.05),而后两地钉螺死亡率差异无显著性(P>0.05)。结论当地钉螺与日本血吸虫不相容可能是宁国市有螺无病地区形成的主要原因之一。
Objective To study the compatibility of Oncomelania japonica and Schistosoma japonicum in snail-free areas in Ningguo, Anhui Province, and to explore the reasons for the formation of snail-free disease. Methods Six hundred snails were collected from the snail-free areas in Ningguo City. They were randomly divided into two groups (n = 300). In the laboratory conditions to the province of Schistosoma japonicum mirabilis infection snail population, the two groups of snails and miracidosis were 1:20 and 1:40. The same method was used to infect the rib snails in Tongling County and the snails in Jingxian County as control. Infected snails placed in the indoor temperature of 22 ~ 24 ℃ feeding 90d, observe the infection rate and death of snails in each group. Results In the 1:20 group and the 1:40 group, snails were not successfully infected by Schistosoma japonicum in the endemic areas of Ningguo, but all the snails were successfully infected in the snail of the rib crusts and Jingxian county in Tongling County. By the end of the observation period, both the natural and the post-infection mortality rates were significantly lower in Tongling County than in Snail and Jingxian County (P <0.05), and then There was no significant difference in the mortality of Oncomelania snails between the two sites (P> 0.05). Conclusion The incompatibility of local snails with Schistosoma japonicum may be one of the main reasons for the formation of snail-free disease in Ningguo.