我们于1979年报告了快速检查痢疾病原菌免疫染色法的研究(人民军医(7): 51,1979)之后,近两年进行了菌体免疫膨胀原理的研究,在此基础上结合方法试用中发现的问题,提出几点注意事项,并对此方法作了一些改进,现介绍如下。一、注意事项(一)玻片上抑菌剂的选择由于本法采用粪便标本直接涂片,滴加抗血清不足以使玻片上痢疾杆菌失活,并且夏季腹泻病人不完全是细菌性痢疾(简称菌痢),致病菌在涂片的冲洗过程中,对工作人员及环境的污染是应考虑的。最简单的办法是在涂片的盐水中加入灭菌剂,使其作用后致病菌失活,但又不能使被检细菌的细胞壁 We reported in 1979 the rapid examination of diarrhea pathogenic bacteria immunostaining research (People’s Military Medical (7): 51,1979), the last two years conducted a study of the principle of bacterial immune expansion, combined with the method found in the trial , Put forward a few considerations, and made some improvements to this method, are presented below. First, matters needing attention (A) the choice of antibacterial agents on slides Because this method uses stool specimens directly smear, dropping antisera is not enough to inactivate the dysentery bacilli on the slide, and the summer diarrhea patients are not completely bacterial dysentery Bacillary dysentery), pathogens in the smear rinse process, the staff and the environment should be taken into account. The easiest way is to add a sterilizing agent in the smear of salt water to inactivate the pathogenic bacteria after its action, but it can not make the cell wall of the tested bacteria