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目的运用反相高效液相色谱法(RP-HPLC)同时测定石胆草药材中一个苯乙醇苷成分(PGs)和3个黄酮碳苷成分(FC-GsⅠ、FC-GsⅡ、FC-GsⅢ)的含量。方法研究优化了石胆草药材中主要成分的检测波长和色谱分离条件等,采用ZORBAXSB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-四氢呋喃(10∶1)-0.02%磷酸为流动相,梯度洗脱,检测波长为332 nm,石胆草中4个主要成分得到分离(Rs>1.5),历时45 min。结果 4种成分线性关系良好,r均大于0.999 0,线性范围分别为0.4~3.2μg(PGs)、0.32~2.56μg(FC-GsⅠ)、0.4~3.2μg(FC-GsⅡ)、0.46~3.52μg(FC-GsⅢ),方法平均加样回收率>98.9%,相对标准偏差RSD<2.08%(n=6)。结论此方法简便、快速、准确,适合用于石胆草药材的质量控制。
OBJECTIVE To determine simultaneously the contents of one phenylethanoid glycoside (PGs) and three flavone glycoside components (FC-GsⅠ, FC-GsⅡ, FC-GsⅢ) in Radix Astragali by RP-HPLC content. The method was used to optimize the detection wavelength and chromatographic separation conditions of the main constituents of Radix Astragali. The chromatographic separation was performed on a ZORBAXSB-C18 (4.6 mm × 250 mm, 5 μm) column with acetonitrile-tetrahydrofuran (10: 1) Mobile phase and gradient elution. The detection wavelength was 332 nm, and the four main components in the chlorella were separated (Rs> 1.5) for 45 min. Results The linear relationship between the four components was good, r> 0.999 0. The linear range was 0.4-3.2μg (PGs), 0.32-2.56μg (FC-GsⅠ), 0.4-3.2μg (FC-GsⅡ) (FC-GsⅢ). The average recoveries were> 98.9% and the relative standard deviation was less than 2.08% (n = 6). Conclusion This method is simple, rapid and accurate, suitable for the quality control of gallbladder herbs.