论文部分内容阅读
近几年来,一些报道表明,在禾本科作物幼胚的愈伤组织中植株再生频率高,例如在玉米、燕麦、高梁、小麦、大麦、珍珠粟。这些愈伤组织还可以用于更进一步的悬浮液和原生质体培养. 这些作者以前是研究水稻幼胚的愈伤组织和悬浮液培养。他们发现在盾片表面以上接种的胚(也就是胚芽一胚根轴的侧面与培养基接触)比盾片表面以下接种的胚对愈伤组织的形成和植株再生作用的效果好。17个水稻品种的测试是一致的;当然。植株再生频率在品种间差异甚大。同时,粳稻类型品种根和芽
In recent years, some reports indicate that the plant regeneration frequency is high in the callus of young embryos of gramineous crops, for example, corn, oats, sorghum, wheat, barley and pearl millet. These callus can also be used for further suspension and protoplast cultures, which were previously cultured in callus and suspension of rice immature embryos. They found that embryos that were inoculated above the surface of the scutellum (ie, the embryo-embryo axis contacts the culture media) have better effects on callus formation and plant regeneration than embryos inoculated below the scutellum surface. The test of 17 rice varieties is consistent; of course. The frequency of plant regeneration varies greatly among cultivars. At the same time, japonica type roots and buds