盐酸如氟抄星(Rufroxacin Hec)为新一代氟喹诺酮类抗菌药。其特点是抗菌谱广,抗菌作用强,长效。其制剂盐如氟沙星片剂应用分光光度法测定其含量获得了满意结果。6份平均回收率为100.18％,RSD为0.80％。 1 仪器与试药 Lambcla 2 分光光度计(美国Perkin Elmer),7520型分光光度计(上海分析厂)。 盐酸如氟沙星对照品和盐酸如氟沙星片(中国药科大学医药化工研究所),盐酸分析纯(南京化学试剂厂)。 2 吸收光谱的绘制 2.1 取盐酸如氟沙星对照品精密称定,加0.1mol/L盐酸液制成每毫升约含10μg的溶液。 2.2 按照处方配比,取适量淀粉、微晶纤维素和滑石粉等制成不含盐酸如氟沙星的模拟溶液。 2.3 取样品适量,用0.1moL/L盐酸液制成每1ml中的含盐酸如氟沙星10μg的溶液,过滤,取续滤液作为样品溶液。 分别取上述溶液,以0.1mol/L盐酸液为空白,绘制 Hydrochloric acid such as Rufroxacin Hec is a new generation fluoroquinolone antibacterial agent. It is characterized by broad antibacterial spectrum, strong antibacterial activity, long-lasting. Satisfactory results were obtained when its formulation salt, such as norfloxacin tablets, was determined spectrophotometrically. The average recoveries of 6 samples were 100.18% with RSD of 0.80%. 1 instruments and reagents Lambcla 2 spectrophotometer (Perkin Elmer, USA), 7520 spectrophotometer (Shanghai Analytical Factory). Hydrochloride such as norfloxacin reference substance and hydrochloric acid such as fluffiatoxin tablets (China Pharmaceutical University Institute of Chemical Industry), hydrochloric acid analytical (Nanjing Chemical Reagent Factory). 2 Absorption Spectrum 2.1 Take hydrochloric acid such as norfloxacin reference standard accurately weighed, add 0.1mol / L hydrochloric acid solution per ml of solution containing about 10μg. 2.2 in accordance with the ratio of prescription, take the right amount of starch, microcrystalline cellulose and talcum powder made of hydrochloric acid solution such as norfloxacin simulated solution. 2.3 take the sample amount, made with 0.1moL / L hydrochloric acid solution per 1ml containing hydrochloric acid such as lofloxacin 10μg, filtered, take the filtrate as the sample solution. Respectively take the above solution to 0.1mol / L hydrochloric acid solution as a blank, draw