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目的 探讨p16和nm2 3 H1在胃癌组织中的表达及其与临床病理因素的关系。 方法 应用免疫组化标记链霉菌抗生物素 生物素 (LSAB)方法 ,对 6 5例胃癌患者癌组织中p16和nm2 3 H1蛋白的表达水平进行了检测 ,并对其结果与患者的临床病理资料进行了相关分析。 结果 p16和nm2 3 H1在胃癌组织中的阳性表达率分别为 30 8%、41 5 % ;在分化好的癌组织 ,p16的阳性表达率高于分化差的癌组织 (P <0 0 5 ) ;p16和nm2 3 H1在无淋巴结转移患者的癌组织中阳性率高于有转移者(P <0 0 5、P <0 0 1) ;在Ⅰ、Ⅱ期患者癌组织中高于Ⅲ期患者癌组织 ,差异均有显著性意义 (P <0 0 5、P <0 0 1)。p16蛋白表达阳性者 3年生存率为 45 0 % ,阴性者为 2 8 9% ;nm2 3 H1蛋白表达阳性者 3年生存率为 5 1 9% ,阴性者为 2 1 1% ,阳性表达者与阴性表达者生存率差异有显著性意义 (P <0 0 5 )。p16与nm2 3 H1蛋白的表达率呈正相关 (r=0 0 415 ,P <0 0 5 )。 结论 p16和nm2 3 H1参与了胃癌的发展过程 ,并与胃癌的部分生物学行为有关 ,可作为预测肿瘤转移和评估患者预后的指标
Objective To investigate the expression of p16 and nm23H1 in gastric cancer and its relationship with clinicopathological factors. Methods The expression of p16 and nm23 H1 protein in the tissues of 65 gastric cancer patients was detected by immunohistochemical method with streptavidin-biotin (LSAB). The results were compared with the clinicopathological data of patients. Correlative analysis was performed. Results The positive expression rates of p16 and nm23H1 in gastric cancer tissues were 30 8% and 415%, respectively. In well-differentiated cancer tissues, the positive rate of p16 expression was higher than that of poorly differentiated cancer tissues (P <0 05). The positive rates of p16 and nm2 3 H1 in cancer tissues without lymph node metastasis were higher than those with metastasis (P < 0.05, P <0 01). In stage I and II cancer tissues, it was higher than that in stage III cancers. There were significant differences in the organization (P <0 05, P <0 01). The 3-year survival rate was 45.0% for patients with positive p16 protein expression and 28.9% for negative; the 3-year survival rate for nm23H1 protein positive was 51.9%, and the negative was 21.11%. There was a significant difference in the survival rate of patients with negative expression (P <0 05). There was a positive correlation between the expression of p16 and nm23H1 protein (r=0 0 415, P <0 05). Conclusions p16 and nm23H1 are involved in the development of gastric cancer and are related to the biological behavior of gastric cancer. They can be used as indicators for predicting tumor metastasis and evaluating the prognosis of patients.