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为研究光触媒抗菌效果评价方法,以霉菌为抗菌对象,用不同载体、培养基和染菌浓度对抗霉效果影响进行了试验观察。结果,在查氏培养基上霉菌生长缓慢,培养周期较长;在马铃薯蔗糖培养基上,各种霉菌生长良好,孢子成熟得快;光触媒抗霉菌试验用无碳源培养时霉菌生长不影响抗菌结果判断。白口铁和PVC作为试验载体影响霉菌生长;以棉布为载体,接种菌量适宜,光触媒的抑菌能力能够较好地体现。以含36 g/L含磷灰石二氧化钛光触媒涂布棉布载体,连续培养28 d,试验组载体上霉菌生长分级为0级,对照组为4级,其抗霉效果较好。结论,光触媒抗霉试验:制备霉菌孢子用马铃薯蔗糖培养基;抗霉试验用培养基选择基础无碳源培养基;使用霉菌孢子悬液浓度为104cfu/m l;试验载体为纯棉布片;采用6 W日光灯照射;试验在温度28℃、相对湿度90%条件下,培养观察14~28 d。
In order to study the method of evaluating the antibacterial effect of photocatalyst, the effect of antimicrobial effect with different carrier, medium and concentration of bacteria was studied by using mold as antibacterial object. As a result, the growth of mold was slow and the culture period was longer in Charcot culture medium. On the potato sucrose medium, various molds grew well and the spores matured rapidly. When the photocatalyst anti-mold test was carried out without carbon source, the mold growth did not affect the antibacterial activity Judgment of the result. White iron and PVC as a test carrier affect the mold growth; cotton as a carrier, appropriate amount of inoculation bacteria, antibacterial ability of the photocatalyst can be better reflected. The cotton carrier was coated with 36 g / L apatite-titania photocatalyst and continuously cultured for 28 days. The mold growth rating of the test group was 0 and the control group was 4, the antimicrobial effect was better. Conclusion: Photocatalyst mildew test: Preparation of mold spores with potato sucrose medium; Mildew test medium selection of non-carbon source culture medium; using mold spore suspension concentration of 104 cfu / ml; test carrier is pure cotton cloth sheet; 6 W fluorescent lamp irradiation; test at a temperature of 28 ℃, relative humidity of 90% under the conditions of culture observed 14 ~ 28 d.