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本研究探讨双色双融合荧光原位杂交技术(DC-DF-FISH)在慢性髓系白血病(CML)中的应用价值,应用常规R-显带技术、DC-DF-FISH、RT-PCR技术检测41例CML患者的染色体核型及bcr/abl融合基因。结果表明,对于初诊CML的18例患者,R-显带显示Ph染色体阳性检出率为94.4%(17/18),DC-DF-FISH阳性检出率也为94.4%(17/18),对于治疗后CML的18例患者,R-显带显示14例有可分析分裂相,其中有11例存在Ph染色体,阳性检出率为78.6%(11/14);而用DC-DF-FISH检测治疗后患者的阳性率为94.4%(17/18);移植后的5例患者R-显带均未检出Ph染色体,而FISH检测出1例bcr/abl基因阳性,RT-PCR证实了FISH的检测结论,但在移植患者中,RT-PCR无阳性发现。结论:双色双融合荧光原位杂交技术具有高度的准确性、可靠性,是检测CML患者bcr/abl基因重排的可靠方法,适用于CML的诊断、疗效判定及微小残留病灶的检测。
This study was to investigate the value of dual-color double-fusion fluorescence in situ hybridization (DC-DF-FISH) in chronic myelogenous leukemia (CML). The expression of DC-DF-FISH was detected by conventional R- Chromosomal Karyotypes and bcr / abl Fusion Genes in 41 CML Patients. The results showed that the positive rate of Ph chromosome was 94.4% (17/18) and the positive rate of DC-DF-FISH was 94.4% (17/18) in 18 patients with newly diagnosed CML, Of the 18 patients with CML after treatment, R-banding revealed 14 cases with analyzable cleavage phase, of which 11 cases had Ph chromosome, the positive detection rate was 78.6% (11/14); while DC-DF-FISH The positive rate of detection after treatment was 94.4% (17/18). No Ph chromosome was detected in R-banding of 5 cases after transplantation, while 1 case of bcr / abl gene was positive by FISH. RT-PCR confirmed FISH test results, but in transplanted patients, no positive RT-PCR was found. CONCLUSION: The dual-color double-fusion fluorescence in situ hybridization is highly accurate and reliable and is a reliable method to detect the gene rearrangement of bcr / abl in CML patients. It is suitable for the diagnosis of CML, the determination of therapeutic effect and the detection of minimal residual lesions.