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严防杂菌污染是菌种生产中的一个重要环节。用化学药物灭菌效果虽好,但易污染空气,危害人体和菌种,而且成本较高。近年来,我们采用蒸汽接种法解决了这个问题,在省内、外一些单位推广使用,反映较好。原理和要点水加热至沸腾产生蒸汽,这种低压蒸汽虽没有多大的杀菌作用,但它是无菌的,尤其是在它膨涨腾升、扩散时产生的压力,能把含有杂菌孢子的自然空气排开,在蒸汽发生器的上面形成一个小范围的无菌区,在这个无菌区内开塞、接种、封塞,就叫做蒸汽接种法。
To prevent bacterial contamination is an important part of the production of bacteria. Sterilization with chemical drugs is good, but easy to pollute the air, endangering the human body and species, and the higher the cost. In recent years, we adopted the steam inoculation method to solve this problem. Some units in the province and outside China promoted their use and reflected better. Principle and point of water heated to boiling to produce steam, although the low-pressure steam is not much bactericidal effect, but it is sterile, especially in its swelling, proliferation pressure, can contain spores of bacteria Natural air lined up in the steam generator to form a small area of sterile, open sterile plug in this area, vaccination, blocking, called steam inoculation.