论文部分内容阅读
目的:观察骨髓间充质(MSCs)干细胞捕获支架能否预防和减少犬冠状动脉支架植入术后再狭窄。方法:①用乙基纤维素作为包被底物对支架进行包被,然后再包被骨髓间充质干细胞抗体。②以球囊损伤法建立犬冠状动脉狭窄模型,应用标准球囊导管技术,将包被有抗体的支架植入犬(n=20)冠状动脉前将支损伤段远端,再以相同方法在损伤段近端植入裸支架作为对照组(n=20),4w后处死动物,取出支架段血管行血管内超声(IVUS)及血管形态学检测分析血管管腔面积和内膜增生面积。结果:术后4周,骨髓间充质干细胞捕获支架组较裸支架组的最小管腔直径和新生内膜面积较明显减少(P<0.05),而最小管腔面积则明显增加(P<0.05)。结论:骨髓间充质干细胞捕获支架能快速修复损伤的血管内膜,降低再狭窄的发生。
Objective: To observe whether bone marrow mesenchymal stem cell (MSCs) capture scaffold can prevent and reduce restenosis after coronary stenting. Methods: ① The scaffolds were coated with ethyl cellulose as coating substrate, and then coated with bone marrow-derived mesenchymal stem cells antibody. (2) The model of canine coronary artery stenosis was established by balloon injury. By using the standard balloon catheter technique, the stent-coated dogs (n = 20) The bare scaffolds were implanted into the proximal end of the injured segment as the control group (n = 20). After 4 weeks, the animals were sacrificed and the intravascular ultrasound (IVUS) and vessel morphological examination were performed to analyze the vessel lumen area and intimal hyperplasia area. Results: After 4 weeks, the smallest lumenal diameter and neointimal area of bone marrow mesenchymal stem cells group were significantly decreased (P <0.05), while the smallest lumen area was significantly increased (P <0.05 ). Conclusion: The bone marrow mesenchymal stem cell-capture scaffold can rapidly repair the injured intima and reduce the occurrence of restenosis.