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本研究应用放射免疫测定法分别测定了甘油致家兔急性肾功能衰竭(ARF)后24、48、72h血浆中TXB_2、6-Keto-PGF_(1α)及6-Keto-PGF_(1α)/TXB_2比值,发现ARF后血浆TXB_2的含量显著升高(P<0.01),6-Keto-PGF_(1α)的含量无明显变化(P>0.05),6-Keto-PGF_(1α)/TXB_2比值显著下降(P<0.01)。应用活血化瘀中药川芎防治ARF能降低血浆中TXB_2含量,使血浆中6-Keto-PGF_(1α)的含量略有升高,6-Keto-PGF_(1α)/TXB_2比值保持在正常水平,提示川芎能够有效地抑制肾缺血后体内血小板的激活,纠正循环血中PGI_2/TXA_2平衡失调,对ARF具有积极的防治作用。
In this study, radioimmunoassay was used to determine plasma levels of TXB_2, 6-Keto-PGF_(1α) and 6-Keto-PGF_(1α)/TXB_2 at 24, 48 and 72 hours after acute renal failure (ARF) in rabbits induced by glycerol. Ratio, found that plasma TXB_2 content increased significantly after ARF (P <0.01), 6-Keto-PGF_ (1α) content did not change significantly (P> 0.05), 6-Keto-PGF_ (1α) / TXB_2 ratio decreased significantly (P<0.01). The use of traditional Chinese medicine Chuanxiong to prevent and treat ARF can reduce TXB_2 content in plasma, increase the content of 6-Keto-PGF_(1α) in plasma, and keep the ratio of 6-Keto-PGF_(1α)/TXB_2 at normal level. Chuanxiong can effectively inhibit the activation of platelets in renal ischemia, and correct the imbalance of PGI_2/TXA_2 in circulating blood, which has a positive effect on prevention and treatment of ARF.