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Objective: To analyze and discuss cell cycles correlation of γ-H2AX, so as to accumulate the data for the further studies of γ-H2AX. Methods: MOLT-4 cells, and peripheral blood lymphocytes (PBLs), with or without 48 h stimulation of phytohemagglutinin (PHA), were irradiated by ultraviolet rays (UV rays). Fluorescence-labeled γ-H2AX antibody was used to detect γ-H2AX foci at the DNA double-strand breaks (DSBs) in chromatin, DNA damage was analyzed by flow cytometry, cell cycle and cell apoptosis were detected by sub-G1 peak method, the expression of y-H2AX was detected by Western blot. Results: With the progression of time, sub-G1 peak emerged apparently in the DNA histograms, and the cells of apoptosis increased gradually; with the progression of time, the increase of γ-H2AX emerged and firstly raised, then decreased; PBLs with 48 h stimulation of PHA entered apparently cell cycle, cells of S and G2/M phase emerged, and PBLs without stimulation of PHA did not enter cell cycle; Western blot showed the increase of the expression of γ-H2AX, and the increase also firstly raised, then decreased. Conclusion: γ-H2AX expressed in the cells of stationary phase and proliferative phase, and with the progression of time, the increase of γ-H2AX firstly raised, and then decreased.