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二乙氨基二硫代甲酸盐(Diethyldithiocarbamate,简称DDC)以剂量1000mg/kg 于照前半小时以10g 体重0.1ml 量腹腔注射给FI 杂种(C57BI×BALB/c)和C3H/km 种小鼠。肿瘤模型是用RIF-1肿瘤细胞10~5皮下注射给16到18周龄的雄鼠(C3H/km)腹侧,在肿瘤体积达150～200mm~3(直径7～8 mm)时给DDC 并进行照射。照射用300kVp X 线机,出光率47拉德/分,全身照射,在照射前或照射时呼吸氮气(含5.5%氧气)5分钟以造成缺氧。呼吸空气的小鼠为有氧照射。照后观察小鼠LD_(50/30),骨髓细胞CFU-S 及肿瘤细胞存活率。照后立即杀死荷瘤小鼠,剥取肿瘤,制备悬液进行活细胞计数,悬液适当稀释后置Waymouth 培
Diethyldithiocarbamate (DDC) was intraperitoneally injected at a dose of 1000 mg/kg in a dose of 0.1 ml in 10 g body weight half an hour before illumination to FI hybrids (C57BI×BALB/c) and C3H/km mice. The tumor model was injected subcutaneously with 10 to 5 RIF-1 tumor cells into the ventral side of 16 to 18-week-old male mice (C3H/km) to give DDC when the tumor volume reached 150 to 200 mm~3 (diameter 7 to 8 mm). And irradiation. The irradiation was performed with a 300 kV X-ray machine, a light emission rate of 47 rad/min, total body irradiation, and nitrogen (including 5.5% oxygen) was taken before or during irradiation for 5 minutes to cause hypoxia. The mice that breathe air are exposed to oxygen. Observe the survival rate of mouse LD_(50/30), bone marrow cells CFU-S and tumor cells. Immediately after the killing of the tumor-bearing mice, the tumors were dissected, a suspension was prepared for viable cell counts, and the suspension was appropriately diluted to prepare Waymouth cultures.