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目的:优选酸荔枝核中抗乙肝黄酮类化合物的提取工艺。方法:以芦丁为对照品,采用UV测定总黄酮含量。以总黄酮提取率为指标,温度、粉碎度、乙醇体积分数、料液比、提取时间、搅拌速度为自变量,在单因素试验基础上,通过Plackett-Burman试验筛选显著影响因子,利用Box-Behnken设计考察各自变量与响应值之间的关系,模拟得到二次多项式回归方程的预测模型,确定最佳提取工艺。结果:粉碎度和乙醇体积分数是影响荔枝核中总黄酮提取效率的显著因子,最佳提取工艺条件为粉碎度120目,加14倍量75%乙醇于60℃提取2 h,搅拌速度90 r·min-1;总黄酮平均提取率17.49%(RSD0.63%)。结论:优选的提取工艺稳定可行,适用于酸荔枝核中抗乙肝总黄酮类化合物的工业化生产。
Objective: To optimize the extraction of anti-hepatitis B flavonoids from sour litchi nucleus. Methods: Rutin was used as reference substance, and the content of total flavonoids was determined by UV. Based on the single factor test, significant influencing factors were screened by the Plackett-Burman test using the extraction rate of total flavonoids as the index, the temperature, the degree of disintegration, the volume fraction of ethanol, the solid-liquid ratio, the extraction time and the stirring speed as independent variables. Behnken designed to investigate the relationship between the respective variables and response values, and simulated the prediction model of quadratic polynomial regression equation to determine the best extraction process. Results: The degree of disintegration and the volume fraction of ethanol were the most significant factors influencing the extraction efficiency of total flavonoids in litchi seedlings. The optimum conditions for the extraction were 120 mesh, 70% ethanol for 14 h and 60 ℃ for 2 h. The stirring speed was 90 r · Min-1; The average extraction rate of total flavonoids was 17.49% (RSD 0.63%). Conclusion: The optimal extraction process is stable and feasible, suitable for the industrial production of anti-hepatitis B total flavonoids in sour litchi nucleus.