论文部分内容阅读
目的：探讨低剂量辐射（LDR）对荷瘤机体巨噬细胞（Mφ）内白细胞介素I（IL—1）和肿瘤坏死因子（TNF）mRNA转录水平的影响。方法：采用C(57)BL／6J小鼠右后肢腓肠肌内接种Lewis肺癌细胞做为实验动物模型，在荷瘤后10d给予75mGyX射线全身照射，照射后18h冲洗腹腔，用贴壁法分离MФ，应用原位杂交组织化学方法检测MФ内IL—1β和TNFa的mRNA转录水平，结果用病理图像密度扫描仪测定的平均密度值（MA）表示。结果：照射组小鼠的MФ内阳性颗粒数明显增多，染色加深；照射组IL—1β和TNFα阳性颗粒的MA值分别为：0．285士0．005、0．272士0·012，假照组分别为：0．216土0．015、0．204士0．005，二者相比具有显著的差异（P＜0．01）。结论:LDR可明显增高荷瘤小鼠MФ的IL—1β和TNFαmRNA转录水平，促进二者的直接抑瘤和免疫调节效应，从而增强了荷瘤机体的免疫功能和杀灭肿瘤细胞的功能。
Objective: To investigate the effect of low-dose radiation (LDR) on the transcription of interleukin (IL-1) and tumor necrosis factor (TNF) mRNA in tumor-bearing macrophages (Mφ). METHODS: Lewis lung cancer cells were inoculated intramuscularly into the right hind limb of C(57)BL/6J mice as an experimental animal model. Whole body irradiation with 75mGy X-rays was performed 10 days after tumor implantation. Abdominal cavity was washed 18h after irradiation, and MФ was isolated by adherent method. The in situ hybridization histochemical method was used to detect the mRNA transcription levels of IL-1β and TNFa in the MФ, and the results were expressed as mean density values (MA) measured by pathological image density scanner. RESULTS: The number of positive particles in the M group was significantly increased and the staining was deeper in the irradiated group; the MA values of IL-1β and TNFα positive particles in the irradiated group were: 0.285 ± 0.005, 0.272 ± 0.012, respectively. Photographic groups were: 0.216 soil 0.015, 0.204 person 0.005, there was a significant difference between the two (P <0.01). Conclusion: LDR can significantly increase the transcriptional level of IL-1β and TNFα mRNA in MФ tumor-bearing mice and promote the direct anti-tumor and immunomodulatory effects of LDR, which enhances the immune function of tumor-bearing mice and kills tumor cells.