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目的:探讨清胰汤改善大鼠急性坏死性胰腺炎(acute necrotizing pancreatitis)ANP炎症反应及肠道通透性功能的治疗效果及机制。方法:将72只雄性SD大鼠随机分为3组,其中2组大鼠采用从胰腺被膜下多点缓慢均匀注入3.8%牛黄胆酸钠(0.5ml/100g)建立大鼠急性坏死性胰腺炎模型,再分为急性坏死性胰腺炎常规治疗组(A组)、清胰汤干预治疗组(B组),其他24只大鼠为假手术组(S组),每组再随机分为24h、48h、72h组。各组于12h后给于肠内营养,B组肠内营养后给于2次清胰汤2.5ml/100g,A组、S组给于同等剂量生理盐水。各组于建模后24h、48h、72h处死,腹腔动脉取血检测血清淀粉酶浓度、IL-6、IL-10、D-乳酸水平。结果:48h时点B组IL-10水平较A组高(P<0.05);72时点B组血清淀粉酶水平较A组低(P<0.01),IL-6水平较A组低(P<0.01),IL-10水平较A组高(P<0.01),D-乳酸水平较A组低(P<0.01)。结论:清胰汤可以上调IL-10改善大鼠急性胰腺炎炎症反应从而降低肠道通透性。
Objective: To investigate the therapeutic effect and mechanism of Qingyi Decoction on inflammatory reaction and intestinal permeability of acute necrotizing pancreatitis in rats. Methods: Seventy-two male Sprague-Dawley rats were randomly divided into 3 groups. Rats in both groups were injected slowly with 3.8% sodium taurocholate (0.5ml / 100g) from the pancreas under the capsule to establish acute necrotic pancreatitis (Group A) and Qingyitang intervention group (group B). The other 24 rats were sham operation group (group S), and each group was randomly divided into 24 hours , 48h, 72h group. Enteral nutrition was given to each group 12h later. After enteral nutrition in group B, 2.5ml / 100g of 2Qiyi Decoction was given to group A, and S group was given the same dose of normal saline. Serum amylase concentration, IL-6, IL-10, and D-lactate levels were measured in all groups after 24h, 48h, 72h after modeling. Results: At 48h, the level of IL-10 in group B was higher than that in group A (P <0.05); The level of serum amylase in group B was lower (P <0.01) and the level of IL-6 was lower <0.01). The level of IL-10 was higher in group A than that in group A (P <0.01). The level of D-lactate in group A was lower than that in group A (P <0.01). Conclusion: Qingyitang can upregulate IL-10 to improve the acute pancreatitis inflammatory response and thus reduce intestinal permeability.