Objective: Gallbladder carcinoma was one of the malignant tumors in the digestive system, characterized by high recurrence and invasion. Recent research indicates that chemotactic factors such as IL-8, MCP-1 and MIP-1α have played an important role in such aspects as formulation, growth, shifting of the tumor. The aim of this study was to investigate expressions of IL-8, MCP-1 and MIP-1α in gallbladder adenocarcinoma tissues. Methods: Gallbladder adenocarcinoma and noncancerous tissues were routinely formalin-fixed and paraffin-embedded, and in situ hybridization assay for IL-8, MCP-1and MIP-1α mRNA. Results:(1) The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA were significantly higher in human gallbladder adenocarcinoma than those in human chronic cholecystitis(P < 0.01). The positive rates or the scorings of three factors were lower in well-differentiatiated gallbladder adenocarcinoma than in poorly-differenfiatted ones, whereas there was only one significant difference between MCP-1 mRNA(P < 0.05). The closely positive correlation were found among IL-8, MCP-1 and MIP-1α mRNA.(2) Both the positive rates of IL-8 mRNA and MCP-1 mRNA as well as their scorings were tightly related to their invasion of the common bile duct and the occurrence of lymph node transfer, moreover, the positive rates of MIP-1α mRNA and its scorings were tightly related to its invasion of liver.(3) Close positive correlation exists not only in IL-8 mRNA and MCP-1 mRNA(r = 0.528), but also in MIP-1α mRNA and IL-8 mRNA(r = 0.422), so does in MCP-1mRNA and MIP-1α mRNA(r = 0.638). Conclusion: The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA are significantly higher in human gallbladder adenocarcinoma than those in human chronic cholecystitis, and the closely positive correlation are found among them, which suggests that IL-8, MCP-1 and MIP-1α regulate and influence the development and transformation of the gallbladder adencarcinoma together. Objective: Gallbladder carcinoma was one of the malignant tumors in the digestive system, characterized by high recurrence and invasion. Recent research indicates that chemotactic factors such as IL-8, MCP-1 and MIP-1α have played an important role in such aspects as formulation, growth, shifting of the tumor. The aim of this study was to investigate expressions of IL-8, MCP-1 and MIP-1α in gallbladder adenocarcinoma tissues. Methods: Gallbladder adenocarcinoma and noncancerous tissues were routinely formalin-fixed and paraffin- embedded, and in situ hybridization assay for IL-8, MCP-1 and MIP-1α mRNA. Results: (1) The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA were significantly higher in human gallbladder adenocarcinoma than those in human chronic cholecystitis (P <0.01). The positive rates or the scorings of three factors were lower in well-differentiatiated gallbladder adenocarcinoma than in poorly-differented ones, there was only one significant differen (2) Both the positive rates of IL-8 mRNA and MCP-1 mRNA as mRNA (P <0.05) well as their scorings were tightly related to their invasion of the common bile duct and the occurrence of lymph node transfer, moreover, the positive rates of MIP-1α mRNA and its scorings were tightly related to its invasion of liver. (3) Close positive correlation was only in IL-8 mRNA and MCP-1 mRNA (r = 0.528), but also in MIP-1α mRNA and IL-8 mRNA (r = 0.422), so does in MCP-1 mRNA and MIP- r = 0.638). Conclusion: The positive rates or the scorings of IL-8, MCP-1 and MIP-1α mRNA are more in human in chronic cholecystitis, and the closely positive correlation are found among them, which suggests that IL-8, MCP-1 and MIP-1α regulate and influence the development and transformation of the gallbladder adencarcinoma together.