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研究报道了制备禾谷镰孢（ＦｕｓａｒｉｕｍｇｒａｍｉｎｅａｒｕｍＳｃｈｗ．）原生质体的方法及影响原生质体再生的条件。ＰＤＳ培养液中培养的菌丝和５％绿豆汁中产生的分生孢子，在２８℃下通过蜗牛酶（３％）、蜗牛酶＋纤维素酶＋溶菌酶（１．５％＋１．５％＋１．５％）酶解，２ｈ开始有原生质体释放，５ｈ左右达释放高峰，后趋于稳定。而以纤维素酶（３％）、溶菌酶（３％）、蜗牛酶＋纤维素酶（１．５％＋１．５％）、蜗牛酶＋溶菌酶（１．５％＋１．５％）、纤维素酶＋溶菌酶（１．５％＋１．５％）处理，原生质体形成效果较差。原生质体在ＰＤＡＳ高渗培养基上再生频率较高（２０％～３０％），在ＭＭＳ上再生频率较低，而在ＮＭＳ上原生质体则不能再生。在ＰＤＡＳ中加入ＫＣｌＯ３和ＭＮＮＧ对原生质体再生有一定影响
This paper reports the preparation of protoplasts from Fusarium graminearum and the conditions affecting the regeneration of protoplasts. Conidia produced in PDS medium and conidia produced in 5% mung bean juice were hydrolyzed by snail enzyme (3%), snail enzyme + cellulase + lysozyme (1.5% + 1.5% + 1.5%) enzymatic hydrolysis, 2h protoplast release, up to about 5h release peak, after tended to be stable. However, cellulase (3%), lysozyme (3%), snail enzyme + cellulase (1.5% + 1.5%), snail enzyme + lysozyme (1.5% + 1.5% Cellulase + lysozyme (1.5% + 1.5%) treatment, protoplast formation less effective. Protoplasts regenerated more frequently on PDAS hypertonic medium (20% -30%), with lower regeneration frequency on MMS, whereas on NMS, protoplasts were not regenerated. The addition of KClO3 and MNNG to PDAS had some effects on the regeneration of protoplasts