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目的 研究附子的抗衰老作用及其作用机制。方法 以 Wister雌性大鼠为研究对象 ,测定青年及老年组大鼠血清总抗氧化能力(TAA)、红细胞超氧化物歧化酶 (SOD)活性、肝组织丙二醛 (MDA)、脑组织脂褐素 (L PF)、心肌组织 Na+ - K+ - ATP酶 (Na+ - K+ - ATPase)及肝细胞膜脂流动性 (LFU)的变化 ;同时观察附子对老年大鼠上述指标的影响。结果 雌性大鼠血清 TAA、红细胞 SOD活性随增龄降低 ;肝组织 MDA和脑组织 LPF含量随增龄升高 ;心肌组织 Na+ - K+ - ATPase随龄无显著变化 ;肝细胞膜 LFU随龄有下降趋势。附子能提高老年大鼠血清 TAA及红细胞SOD的活性 ,降低脑组织 LPF和肝组织 MDA含量 ,增加心肌组织 Na+ - K+ - ATPase的活性 ,可改善肝细胞膜 L FU。在给附子 1 0、2 0和 30 d后测定以上各指标 ,发现附子发挥作用的最佳时间为 30 d。结论 附子能增强机体抗氧化能力 ,具有抗衰老作用
Objective To study the anti-aging effects of Aconite and its mechanism of action. Methods Wistar female rats were used as subjects. The serum total antioxidant capacity (TAA), erythrocyte superoxide dismutase (SOD) activity, liver malondialdehyde (MDA), and brain tissue lipid brown were measured in young and old rats. LPF, myocardial Na+-K+-ATPase (Na+-K+-ATPase) and hepatocyte membrane lipid fluidity (LFU) were observed. The effects of aconite on the above indexes in aged rats were also observed. Results Serum TAA and red blood cell SOD activity in female rats decreased with increasing age; MDA and LPF content in brain tissue increased with age; Na+-K+-ATPase in cardiac tissue did not change significantly with age; LFU in hepatocyte membrane decreased with age. . Aconite can increase the activity of serum TAA and erythrocyte SOD, decrease the content of MDA in brain tissue and liver tissue, increase the activity of Na+-K+-ATPase in cardiac tissue, and improve L FU in hepatocyte membrane. The above indexes were measured after 10, 20 and 30 days of aconite, and the best time for aconite to function was 30 days. Conclusion Aconite can enhance the body’s antioxidant capacity and has anti-aging effects.